Urine cytology and the diagnosis of renal allograft rejection: II. Studies using immunostaining

Howard E. Corey, Flores Alfonso, Diane Hamele-Bena, Stuart M. Greenstein, Richard Schechner, Vivian Tellis, Pinhas Geva, Leopold G. Koss

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

BACKGROUND: Urine immunocytology may provide a noninvasive method of investigating the antigens expressed by renal tubular cells. In previous investigations of patients with acute renal allograft rejection (AR), we showed that the adhesion molecule ICAM-1 is expressed by voided tubular cells. The up-regulation of roll ICAM-1, in turn, may be due to high circulating levels of interferon-gamma and/or TNF-α. We investigated the regulation of receptors for these cytokines and found a Correlation between their expression and clinical events. STUDY DESIGN: For 10 patients who received transplants consecutively, freshly voided aliquots of urine were obtained on each hospital day and on each outpatient visit for a mean of 52.8 ± 26.2 (SD) days. After cytocentrifugation, the samples were prepared by the avidin - biotin - immunoperoxidase technique in order to detect the presence or absence of ICAM-1, interferon-gamma receptor and TNF-α receptor (p 80) on the tubular cells. RESULTS: In nonrejecting patients, the tubular cells expressed the interferon-gamma receptor but not ICAM-1 or the TNF-α receptor. In patients with AR, the pattern was different. The tubular cells eXpressed ICAM-1 and the TNF-α receptor but not the interferon-gamma receptor. CONCLUSION: Urine immunocytochemistry may be useful to demonstrate the expression of cytokine receptors by renal epithelia.

Original languageEnglish (US)
Pages (from-to)1742-1746
Number of pages5
JournalActa Cytologica
Volume41
Issue number6
StatePublished - 1997

Fingerprint

Intercellular Adhesion Molecule-1
Allografts
Cell Biology
Urine
Tumor Necrosis Factor Receptors
Kidney
Cytokine Receptors
Avidin
Biotin
Immunoenzyme Techniques
Interferon-gamma
Outpatients
Up-Regulation
Epithelium
Immunohistochemistry
Transplants
interferon gamma receptor

Keywords

  • Allograft
  • Immunocytochemistry
  • Kidney transplantation
  • Transplant rejection
  • Urine

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology
  • Histology

Cite this

Corey, H. E., Alfonso, F., Hamele-Bena, D., Greenstein, S. M., Schechner, R., Tellis, V., ... Koss, L. G. (1997). Urine cytology and the diagnosis of renal allograft rejection: II. Studies using immunostaining. Acta Cytologica, 41(6), 1742-1746.

Urine cytology and the diagnosis of renal allograft rejection : II. Studies using immunostaining. / Corey, Howard E.; Alfonso, Flores; Hamele-Bena, Diane; Greenstein, Stuart M.; Schechner, Richard; Tellis, Vivian; Geva, Pinhas; Koss, Leopold G.

In: Acta Cytologica, Vol. 41, No. 6, 1997, p. 1742-1746.

Research output: Contribution to journalArticle

Corey, HE, Alfonso, F, Hamele-Bena, D, Greenstein, SM, Schechner, R, Tellis, V, Geva, P & Koss, LG 1997, 'Urine cytology and the diagnosis of renal allograft rejection: II. Studies using immunostaining', Acta Cytologica, vol. 41, no. 6, pp. 1742-1746.
Corey HE, Alfonso F, Hamele-Bena D, Greenstein SM, Schechner R, Tellis V et al. Urine cytology and the diagnosis of renal allograft rejection: II. Studies using immunostaining. Acta Cytologica. 1997;41(6):1742-1746.
Corey, Howard E. ; Alfonso, Flores ; Hamele-Bena, Diane ; Greenstein, Stuart M. ; Schechner, Richard ; Tellis, Vivian ; Geva, Pinhas ; Koss, Leopold G. / Urine cytology and the diagnosis of renal allograft rejection : II. Studies using immunostaining. In: Acta Cytologica. 1997 ; Vol. 41, No. 6. pp. 1742-1746.
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AU - Schechner, Richard

AU - Tellis, Vivian

AU - Geva, Pinhas

AU - Koss, Leopold G.

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