Uptake of enalapril and expression of organic anion transporting polypeptide 1 in zonal, isolated rat hepatocytes

Tawfic Nessim Abu-Zahra, Allan W. Wolkoff, Richard B. Kim, K. Sandy Pang

Research output: Contribution to journalArticle

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Abstract

Sinusoidal entry is the first obligatory process preceding intracellular drug removal in liver. Transport of the angiotensin converting enzyme inhibitor enalapril (1-750 μM with [3H]enalapril), a substrate of Oatp1, the sodium-independent organic anion transporting polypeptide 1 cloned from rat liver, was studied in rat hepatocytes isolated from all zones of the liver (homogeneous) and from enriched periportal (PP) and perivenous (PV) hepatocytes prepared by collagenase perfusion and zone-selective destruction with digitonin, respectively. Uptake was linear over 1 rain and was concentration-dependent. Transport by the homogeneous hepatocytes (in the presence and absence of Na+) and PP and PV cells was described by single saturable components of similar kinetic constants (K(m) values of 344-461 μM and V(max) values of 9.5-11.6 nmol/min/106 cells; P > .05, ANOVA). The K(m) value for enalapril uptake in hepatocytes was of the same order of magnitude compared with that for Oatp1 expressed in HeLa cells transfected with cDNA- Oatp1 and Western blot analysis revealed similar levels of immunoreactive Oatp1 expression in PP and PV hepatocytes. However, enalapril was not taken up by Oatp2 nor by the human OATP expressed in recombinant vaccinia systems.

Original languageEnglish (US)
Pages (from-to)801-806
Number of pages6
JournalDrug Metabolism and Disposition
Volume28
Issue number7
StatePublished - 2000

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Enalapril
Anions
Rats
Hepatocytes
Peptides
Liver
Vaccinia
Digitonin
Rain
Collagenases
Analysis of variance (ANOVA)
HeLa Cells
Angiotensin-Converting Enzyme Inhibitors
Analysis of Variance
Complementary DNA
Perfusion
Western Blotting
Sodium
Kinetics
Substrates

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Uptake of enalapril and expression of organic anion transporting polypeptide 1 in zonal, isolated rat hepatocytes. / Abu-Zahra, Tawfic Nessim; Wolkoff, Allan W.; Kim, Richard B.; Pang, K. Sandy.

In: Drug Metabolism and Disposition, Vol. 28, No. 7, 2000, p. 801-806.

Research output: Contribution to journalArticle

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