Up-regulation of MAP2e-expressing oligodendrocytes in the white matter of patients with HIV-1 encephalitis

Melissa A. Cosenza, Meng Liang Zhao, Sai L. Shankar, Bridget Shafit-Zagardo, Sunhee C. Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

HIV-1 encephalitis (HIVE) is characterized by infection of macrophages and microglial cells, diffuse gliosis, and damage to neuronal populations. The nature of the white matter damage in HIVE remains elusive, and little is known about the status of the oligodendrocyte in HIVE. We have recently described a novel isoform of microtubule-associated protein-2 (MAP2e), which is expressed transiently in developing oligodendrocytes during myelination, and in remyelinating oligodendrocytes in multiple sclerosis lesions. In this study, we tested the hypothesis that MAP2e expression would be increased in the white matter of HIVE. We analysed brain sections from patients with HIVE and controls (HIV+ and HIV-) by immunocytochemistry and found that MAP2e+ cells are significantly increased in HIVE (range, 5-167 cells per cm2) compared to controls (range, 1-25 cells per cm2). MAP2e+ cells were negative for GFAP, CD68, LN3, RCA-1, von Willebrand factor and HIV-1 p24, but positive for MBP or Luxol-Fast Blue, supporting their oligodendroglial lineage. A topographical association between MAP2e and HIV-1 p24 expression was noted, but not between MAP2e and β-APP, a marker of damaged axons. Our results demonstrate that MAP2e can serve as a marker of white matter damage in HIVE and support the notion that oligodendrocyte damage/repair occurs during HIV-1 infection.

Original languageEnglish (US)
Pages (from-to)480-488
Number of pages9
JournalNeuropathology and Applied Neurobiology
Volume28
Issue number6
DOIs
StatePublished - 2002

Fingerprint

Oligodendroglia
Encephalitis
HIV-1
Up-Regulation
White Matter
HIV
Gliosis
Microtubule-Associated Proteins
von Willebrand Factor
Multiple Sclerosis
HIV Infections
Axons
Protein Isoforms
Immunohistochemistry
Macrophages

Keywords

  • AIDS
  • Microtubule-associated protein
  • Myelin
  • Oligodendrocytes
  • White matter

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Up-regulation of MAP2e-expressing oligodendrocytes in the white matter of patients with HIV-1 encephalitis. / Cosenza, Melissa A.; Zhao, Meng Liang; Shankar, Sai L.; Shafit-Zagardo, Bridget; Lee, Sunhee C.

In: Neuropathology and Applied Neurobiology, Vol. 28, No. 6, 2002, p. 480-488.

Research output: Contribution to journalArticle

Cosenza, Melissa A. ; Zhao, Meng Liang ; Shankar, Sai L. ; Shafit-Zagardo, Bridget ; Lee, Sunhee C. / Up-regulation of MAP2e-expressing oligodendrocytes in the white matter of patients with HIV-1 encephalitis. In: Neuropathology and Applied Neurobiology. 2002 ; Vol. 28, No. 6. pp. 480-488.
@article{eb883b0586194f6ab664099ea9549278,
title = "Up-regulation of MAP2e-expressing oligodendrocytes in the white matter of patients with HIV-1 encephalitis",
abstract = "HIV-1 encephalitis (HIVE) is characterized by infection of macrophages and microglial cells, diffuse gliosis, and damage to neuronal populations. The nature of the white matter damage in HIVE remains elusive, and little is known about the status of the oligodendrocyte in HIVE. We have recently described a novel isoform of microtubule-associated protein-2 (MAP2e), which is expressed transiently in developing oligodendrocytes during myelination, and in remyelinating oligodendrocytes in multiple sclerosis lesions. In this study, we tested the hypothesis that MAP2e expression would be increased in the white matter of HIVE. We analysed brain sections from patients with HIVE and controls (HIV+ and HIV-) by immunocytochemistry and found that MAP2e+ cells are significantly increased in HIVE (range, 5-167 cells per cm2) compared to controls (range, 1-25 cells per cm2). MAP2e+ cells were negative for GFAP, CD68, LN3, RCA-1, von Willebrand factor and HIV-1 p24, but positive for MBP or Luxol-Fast Blue, supporting their oligodendroglial lineage. A topographical association between MAP2e and HIV-1 p24 expression was noted, but not between MAP2e and β-APP, a marker of damaged axons. Our results demonstrate that MAP2e can serve as a marker of white matter damage in HIVE and support the notion that oligodendrocyte damage/repair occurs during HIV-1 infection.",
keywords = "AIDS, Microtubule-associated protein, Myelin, Oligodendrocytes, White matter",
author = "Cosenza, {Melissa A.} and Zhao, {Meng Liang} and Shankar, {Sai L.} and Bridget Shafit-Zagardo and Lee, {Sunhee C.}",
year = "2002",
doi = "10.1046/j.1365-2990.2002.00420.x",
language = "English (US)",
volume = "28",
pages = "480--488",
journal = "Neuropathology and Applied Neurobiology",
issn = "0305-1846",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Up-regulation of MAP2e-expressing oligodendrocytes in the white matter of patients with HIV-1 encephalitis

AU - Cosenza, Melissa A.

AU - Zhao, Meng Liang

AU - Shankar, Sai L.

AU - Shafit-Zagardo, Bridget

AU - Lee, Sunhee C.

PY - 2002

Y1 - 2002

N2 - HIV-1 encephalitis (HIVE) is characterized by infection of macrophages and microglial cells, diffuse gliosis, and damage to neuronal populations. The nature of the white matter damage in HIVE remains elusive, and little is known about the status of the oligodendrocyte in HIVE. We have recently described a novel isoform of microtubule-associated protein-2 (MAP2e), which is expressed transiently in developing oligodendrocytes during myelination, and in remyelinating oligodendrocytes in multiple sclerosis lesions. In this study, we tested the hypothesis that MAP2e expression would be increased in the white matter of HIVE. We analysed brain sections from patients with HIVE and controls (HIV+ and HIV-) by immunocytochemistry and found that MAP2e+ cells are significantly increased in HIVE (range, 5-167 cells per cm2) compared to controls (range, 1-25 cells per cm2). MAP2e+ cells were negative for GFAP, CD68, LN3, RCA-1, von Willebrand factor and HIV-1 p24, but positive for MBP or Luxol-Fast Blue, supporting their oligodendroglial lineage. A topographical association between MAP2e and HIV-1 p24 expression was noted, but not between MAP2e and β-APP, a marker of damaged axons. Our results demonstrate that MAP2e can serve as a marker of white matter damage in HIVE and support the notion that oligodendrocyte damage/repair occurs during HIV-1 infection.

AB - HIV-1 encephalitis (HIVE) is characterized by infection of macrophages and microglial cells, diffuse gliosis, and damage to neuronal populations. The nature of the white matter damage in HIVE remains elusive, and little is known about the status of the oligodendrocyte in HIVE. We have recently described a novel isoform of microtubule-associated protein-2 (MAP2e), which is expressed transiently in developing oligodendrocytes during myelination, and in remyelinating oligodendrocytes in multiple sclerosis lesions. In this study, we tested the hypothesis that MAP2e expression would be increased in the white matter of HIVE. We analysed brain sections from patients with HIVE and controls (HIV+ and HIV-) by immunocytochemistry and found that MAP2e+ cells are significantly increased in HIVE (range, 5-167 cells per cm2) compared to controls (range, 1-25 cells per cm2). MAP2e+ cells were negative for GFAP, CD68, LN3, RCA-1, von Willebrand factor and HIV-1 p24, but positive for MBP or Luxol-Fast Blue, supporting their oligodendroglial lineage. A topographical association between MAP2e and HIV-1 p24 expression was noted, but not between MAP2e and β-APP, a marker of damaged axons. Our results demonstrate that MAP2e can serve as a marker of white matter damage in HIVE and support the notion that oligodendrocyte damage/repair occurs during HIV-1 infection.

KW - AIDS

KW - Microtubule-associated protein

KW - Myelin

KW - Oligodendrocytes

KW - White matter

UR - http://www.scopus.com/inward/record.url?scp=0036444245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036444245&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2990.2002.00420.x

DO - 10.1046/j.1365-2990.2002.00420.x

M3 - Article

VL - 28

SP - 480

EP - 488

JO - Neuropathology and Applied Neurobiology

JF - Neuropathology and Applied Neurobiology

SN - 0305-1846

IS - 6

ER -