Up-regulation of amphotrophic retroviral receptor expression in human peripheral blood CD34+ cells

Andreas Kaubisch, Maureen Ward, Stuti Schoetz, Charles Hesdorffer, Arthur Bank

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Retroviral-mediated gene transfer into hematopoietic stem cells provides the only means of stable transduction of these cells and their progeny for use with a variety of potentially therapeutic genes. Expression of the Moloney amphotropic retroviral receptor - pit-2 or GLVR-2 - is critical to the recognition and entry of Moloney leukemia virus-derived viruses into human target cells such as CD34+ hematopoietic cells. GLVR-2 functions as a sodium-dependent phosphate transporter as well as a receptor. We have previously shown that the expression of the murine homologue of the amphotropic receptor Ram1, also a phosphate transporter, is developmentally regulated in murine hematopoietic fetal liver cells. We also demonstrated that culture of murine fetal liver cells in phosphate-free (PO4-free) medium increases levels of receptor mRNA and makes murine fetal liver cells susceptible to Moloney amphotropic viral gene transfer. We now examine the effect of culture conditions on the expression of GLVR-2 in human CD34+ cells. In this report, we demonstrate that there is a 2-3 fold increase in GLVR-2 mRNA levels in CD34+ cells after 3 days in culture with interleukin 3, interleukin 6, and stem-cell factor. In addition, the use of PO4-free medium increases expression of GLVR-2 an additional 2-fold in these cells during this time. These results indicate that GLVR-2 expression can be up-regulated on these cells, and may permit improved retroviral gene transfer efficiencies.

Original languageEnglish (US)
Pages (from-to)243-253
Number of pages11
JournalAmerican Journal of Hematology
Volume61
Issue number4
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Amphotropic receptor
  • Gene therapy
  • Gene transfer
  • Retroviral

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Up-regulation of amphotrophic retroviral receptor expression in human peripheral blood CD34+ cells'. Together they form a unique fingerprint.

  • Cite this