Unusual DNA binding characteristics of an in vitro translation product of the CCAAT binding protein mYB-1

Xioxia Gai, Kenneth E. Lipson, Michael B. Prystowsky

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have isolated a cDNA that encodes the murine CCAAT-binding protein mYB-1. The deduced amino acid sequence shows 95% identity with its presumed human homologue (hYB-1A) which was originally isolated as a protein that binds to the Y box of MHC class II genes. In vitro translated mYB-1 binds to CCAAT boxes of the MHCIIEα, HSVTK and mouse PCNA promoters but not to α-globin or human thymidine kinase CCAAT boxes. Interestingly, complexes formed between the in vitro translated protein and the various CCAAT boxes display the property of being competed more efficiently with self competitor DNA, regardless of the CCAAT box initially used as a probe. A similar phenomenon was observed in a cell extract of Con-A stimulated murine splenocytes when the same competition assays were performed. These results may reflect the generation of multiple forms of a particular CCAAT-binding protein, such as mYB-1, that display distinct, yet overlapping, DNA binding specificities.

Original languageEnglish (US)
Pages (from-to)601-606
Number of pages6
JournalNucleic Acids Research
Volume20
Issue number3
StatePublished - 1992
Externally publishedYes

Fingerprint

Carrier Proteins
DNA
Proteins
Protein
MHC Class II Genes
Globins
Thymidine Kinase
Proliferating Cell Nuclear Antigen
Cell Extracts
Amino acids
Amino Acid Sequence
Assays
Complementary DNA
Genes
Amino Acids
CDNA
Promoter
Specificity
Overlapping
Mouse

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Unusual DNA binding characteristics of an in vitro translation product of the CCAAT binding protein mYB-1. / Gai, Xioxia; Lipson, Kenneth E.; Prystowsky, Michael B.

In: Nucleic Acids Research, Vol. 20, No. 3, 1992, p. 601-606.

Research output: Contribution to journalArticle

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