TY - JOUR
T1 - Unique antigen of cultured Hodgkin's cells. A putative sialyltransferase
AU - Paietta, E.
AU - Stockert, R. J.
AU - Morell, A. G.
AU - Diehl, V.
AU - Wiernik, P. H.
PY - 1986
Y1 - 1986
N2 - Hodgkin's disease-derived giant cell lines (HD-cells) express high levels of ectosialyltransferase activity presumed to be a galactose-specific lectin recognizing the desialylated 3-fucosyl-N-acetyllactosamine structure (X-hapten). Both the anti-X-hapten monoclonal antibody VIM-D5 and a polyclonal antiserum to another galactose-lectin, the hepatic asialoglycoprotein receptor (HBP), recognize a 55,000-mol wt HD-cell protein (Paietta, E., R.J. Stockert, A.G. Morell, V. Diehl, and P.H. Weirnik. 1986. Proc. Natl. Acad. Sci. USA. 83:3451-3455). That the expression of the 55,000-mol wt protein is restricted to HD-cells among X-hapten positive cell lines is confirmed in this study. The 55,000-mol wt protein is shown to be present on the cell surface and intracellularly, where an additional immunocrossreactive 150,000-mol wt protein is recognized. Extraction of the 55,000 mol wt protein from HD-cell lysates by affinity chromatography results in the loss of sialyltransferase activity. While evidence for a single protein possessing both the antigenic and the enzymatic activity is not direct, these results suggest that the ectosialyltransferase unique to HD-cells is a 55,000-mol wt membrane glycoprotein possessing the X-hapten oligosaccharide.
AB - Hodgkin's disease-derived giant cell lines (HD-cells) express high levels of ectosialyltransferase activity presumed to be a galactose-specific lectin recognizing the desialylated 3-fucosyl-N-acetyllactosamine structure (X-hapten). Both the anti-X-hapten monoclonal antibody VIM-D5 and a polyclonal antiserum to another galactose-lectin, the hepatic asialoglycoprotein receptor (HBP), recognize a 55,000-mol wt HD-cell protein (Paietta, E., R.J. Stockert, A.G. Morell, V. Diehl, and P.H. Weirnik. 1986. Proc. Natl. Acad. Sci. USA. 83:3451-3455). That the expression of the 55,000-mol wt protein is restricted to HD-cells among X-hapten positive cell lines is confirmed in this study. The 55,000-mol wt protein is shown to be present on the cell surface and intracellularly, where an additional immunocrossreactive 150,000-mol wt protein is recognized. Extraction of the 55,000 mol wt protein from HD-cell lysates by affinity chromatography results in the loss of sialyltransferase activity. While evidence for a single protein possessing both the antigenic and the enzymatic activity is not direct, these results suggest that the ectosialyltransferase unique to HD-cells is a 55,000-mol wt membrane glycoprotein possessing the X-hapten oligosaccharide.
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U2 - 10.1172/JCI112583
DO - 10.1172/JCI112583
M3 - Article
C2 - 3734096
AN - SCOPUS:0022521857
SN - 0021-9738
VL - 78
SP - 349
EP - 354
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 2
ER -