Unique antigen of cultured Hodgkin's cells. A putative sialyltransferase

Elisabeth M. Paietta, R. J. Stockert, A. G. Morell, V. Diehl, P. H. Wiernik

Research output: Contribution to journalArticle

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Abstract

Hodgkin's disease-derived giant cell lines (HD-cells) express high levels of ectosialyltransferase activity presumed to be a galactose-specific lectin recognizing the desialylated 3-fucosyl-N-acetyllactosamine structure (X-hapten). Both the anti-X-hapten monoclonal antibody VIM-D5 and a polyclonal antiserum to another galactose-lectin, the hepatic asialoglycoprotein receptor (HBP), recognize a 55,000-mol wt HD-cell protein (Paietta, E., R.J. Stockert, A.G. Morell, V. Diehl, and P.H. Weirnik. 1986. Proc. Natl. Acad. Sci. USA. 83:3451-3455). That the expression of the 55,000-mol wt protein is restricted to HD-cells among X-hapten positive cell lines is confirmed in this study. The 55,000-mol wt protein is shown to be present on the cell surface and intracellularly, where an additional immunocrossreactive 150,000-mol wt protein is recognized. Extraction of the 55,000 mol wt protein from HD-cell lysates by affinity chromatography results in the loss of sialyltransferase activity. While evidence for a single protein possessing both the antigenic and the enzymatic activity is not direct, these results suggest that the ectosialyltransferase unique to HD-cells is a 55,000-mol wt membrane glycoprotein possessing the X-hapten oligosaccharide.

Original languageEnglish (US)
Pages (from-to)349-354
Number of pages6
JournalJournal of Clinical Investigation
Volume78
Issue number2
StatePublished - 1986

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Sialyltransferases
Hodgkin Disease
CD15 Antigens
Giant Cells
Cultured Cells
Antigens
Cell Line
Proteins
Galactose
Lectins
Asialoglycoprotein Receptor
Membrane Glycoproteins
Oligosaccharides
Affinity Chromatography
Immune Sera
Monoclonal Antibodies

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Paietta, E. M., Stockert, R. J., Morell, A. G., Diehl, V., & Wiernik, P. H. (1986). Unique antigen of cultured Hodgkin's cells. A putative sialyltransferase. Journal of Clinical Investigation, 78(2), 349-354.

Unique antigen of cultured Hodgkin's cells. A putative sialyltransferase. / Paietta, Elisabeth M.; Stockert, R. J.; Morell, A. G.; Diehl, V.; Wiernik, P. H.

In: Journal of Clinical Investigation, Vol. 78, No. 2, 1986, p. 349-354.

Research output: Contribution to journalArticle

Paietta, EM, Stockert, RJ, Morell, AG, Diehl, V & Wiernik, PH 1986, 'Unique antigen of cultured Hodgkin's cells. A putative sialyltransferase', Journal of Clinical Investigation, vol. 78, no. 2, pp. 349-354.
Paietta, Elisabeth M. ; Stockert, R. J. ; Morell, A. G. ; Diehl, V. ; Wiernik, P. H. / Unique antigen of cultured Hodgkin's cells. A putative sialyltransferase. In: Journal of Clinical Investigation. 1986 ; Vol. 78, No. 2. pp. 349-354.
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