Unexpected link between metal ion deficiency and autophagy in Aspergillus fumigatus

Daryl L. Richie, Kevin K. Fuller, Jarrod Fortwendel, Michael D. Miley, Jason W. McCarthy, Marta Feldmesser, Judith C. Rhodes, David S. Askew

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Autophagy is the major cellular pathway for bulk degradation of cytosolic material and is required to maintain viability under starvation conditions. To determine the contribution of autophagy to starvation stress responses in the filamentous fungus Aspergillus fumigatus, we disrupted the A. fumigatus atg1 gene, encoding a serine/threonine kinase required for autophagy. The ΔAfatg1 mutant showed abnormal conidiophore development and reduced conidiation, but the defect could be bypassed by increasing the nitrogen content of the medium. When transferred to starvation medium, wild-type hyphae were able to undergo a limited amount of growth, resulting in radial expansion of the colony. In contrast, the ΔAfatg1 mutant was unable to grow under these conditions. However, supplementation of the medium with metal ions rescued the ability of the ΔAfatg1 mutant to grow in the absence of a carbon or nitrogen source. Depleting the medium of cations by using EDTA was sufficient to induce autophagy in wild-type A. fumigatus, even in the presence of abundant carbon and nitrogen, and the ΔAfatg1 mutant was severely growth impaired under these conditions. These findings establish a role for autophagy in the recycling of internal nitrogen sources to support conidiophore development and suggest that autophagy also contributes to the recycling of essential metal ions to sustain hyphal growth when exogenous nutrients are scarce.

Original languageEnglish (US)
Pages (from-to)2437-2447
Number of pages11
JournalEukaryotic Cell
Volume6
Issue number12
DOIs
StatePublished - Dec 2007

Fingerprint

Aspergillus fumigatus
autophagy
Autophagy
metal ions
Metals
Ions
Nitrogen
Starvation
starvation
mutants
conidiophores
recycling
nitrogen
Carbon
Growth
Hyphae
carbon
Protein-Serine-Threonine Kinases
Recycling
threonine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Richie, D. L., Fuller, K. K., Fortwendel, J., Miley, M. D., McCarthy, J. W., Feldmesser, M., ... Askew, D. S. (2007). Unexpected link between metal ion deficiency and autophagy in Aspergillus fumigatus. Eukaryotic Cell, 6(12), 2437-2447. https://doi.org/10.1128/EC.00224-07

Unexpected link between metal ion deficiency and autophagy in Aspergillus fumigatus. / Richie, Daryl L.; Fuller, Kevin K.; Fortwendel, Jarrod; Miley, Michael D.; McCarthy, Jason W.; Feldmesser, Marta; Rhodes, Judith C.; Askew, David S.

In: Eukaryotic Cell, Vol. 6, No. 12, 12.2007, p. 2437-2447.

Research output: Contribution to journalArticle

Richie, DL, Fuller, KK, Fortwendel, J, Miley, MD, McCarthy, JW, Feldmesser, M, Rhodes, JC & Askew, DS 2007, 'Unexpected link between metal ion deficiency and autophagy in Aspergillus fumigatus', Eukaryotic Cell, vol. 6, no. 12, pp. 2437-2447. https://doi.org/10.1128/EC.00224-07
Richie DL, Fuller KK, Fortwendel J, Miley MD, McCarthy JW, Feldmesser M et al. Unexpected link between metal ion deficiency and autophagy in Aspergillus fumigatus. Eukaryotic Cell. 2007 Dec;6(12):2437-2447. https://doi.org/10.1128/EC.00224-07
Richie, Daryl L. ; Fuller, Kevin K. ; Fortwendel, Jarrod ; Miley, Michael D. ; McCarthy, Jason W. ; Feldmesser, Marta ; Rhodes, Judith C. ; Askew, David S. / Unexpected link between metal ion deficiency and autophagy in Aspergillus fumigatus. In: Eukaryotic Cell. 2007 ; Vol. 6, No. 12. pp. 2437-2447.
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