Unexpected expression of glucose transporter 4 in villous stromal cells of human placenta

A. Y. Xing, J. C. Challier, J. Lepercq, M. Caüzac, Maureen J. Charron, J. Girard, S. Hauguel-de Mouzon

Research output: Contribution to journalArticle

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Abstract

Glucose transporter 4 (GLUT4) protein expression was characterized in human and rodent term placentas. A 50-kDa protein was detected, by immunoblotting, in term human placenta at levels averaging 25% of those found in white adipose tissue. It was also present, albeit at lower levels, in mouse and rat placentas. The specificity of the 50-kDa signal was established by using skeletal muscle and placental tissues obtained from GLUT4-null mice as controls. Indirect immunohistochemistry, performed in human placentas, showed that intravillous stromal cells were conspicuously labeled by GLUT4 and revealed colocalization of GLUT4 transporters with insulin receptors. This study provides the first evidence that the insulinresponsive GLUT4 glucose transporter is present in human and rodent hemochorial placentas. Placental GLUT4 gene and protein levels were not modified in human pregnancy complicated by insulin-dependent diabetes mellitus. The significance of the high level of GLUT4 protein in human placenta remains to be elucidated, because, so far, this organ was not considered to be insulin-sensitive, with regard to glucose transport.

Original languageEnglish (US)
Pages (from-to)4097-4101
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume83
Issue number11
StatePublished - 1998

Fingerprint

Facilitative Glucose Transport Proteins
Stromal Cells
Placenta
Cells
Rodentia
Proteins
Insulin
Tissue
White Adipose Tissue
Insulin Receptor
Medical problems
Type 1 Diabetes Mellitus
Immunoblotting
Muscle
Rats
Skeletal Muscle
Immunohistochemistry
Glucose
Pregnancy
Muscles

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Xing, A. Y., Challier, J. C., Lepercq, J., Caüzac, M., Charron, M. J., Girard, J., & Hauguel-de Mouzon, S. (1998). Unexpected expression of glucose transporter 4 in villous stromal cells of human placenta. Journal of Clinical Endocrinology and Metabolism, 83(11), 4097-4101.

Unexpected expression of glucose transporter 4 in villous stromal cells of human placenta. / Xing, A. Y.; Challier, J. C.; Lepercq, J.; Caüzac, M.; Charron, Maureen J.; Girard, J.; Hauguel-de Mouzon, S.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 83, No. 11, 1998, p. 4097-4101.

Research output: Contribution to journalArticle

Xing, AY, Challier, JC, Lepercq, J, Caüzac, M, Charron, MJ, Girard, J & Hauguel-de Mouzon, S 1998, 'Unexpected expression of glucose transporter 4 in villous stromal cells of human placenta', Journal of Clinical Endocrinology and Metabolism, vol. 83, no. 11, pp. 4097-4101.
Xing, A. Y. ; Challier, J. C. ; Lepercq, J. ; Caüzac, M. ; Charron, Maureen J. ; Girard, J. ; Hauguel-de Mouzon, S. / Unexpected expression of glucose transporter 4 in villous stromal cells of human placenta. In: Journal of Clinical Endocrinology and Metabolism. 1998 ; Vol. 83, No. 11. pp. 4097-4101.
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AU - Xing, A. Y.

AU - Challier, J. C.

AU - Lepercq, J.

AU - Caüzac, M.

AU - Charron, Maureen J.

AU - Girard, J.

AU - Hauguel-de Mouzon, S.

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AB - Glucose transporter 4 (GLUT4) protein expression was characterized in human and rodent term placentas. A 50-kDa protein was detected, by immunoblotting, in term human placenta at levels averaging 25% of those found in white adipose tissue. It was also present, albeit at lower levels, in mouse and rat placentas. The specificity of the 50-kDa signal was established by using skeletal muscle and placental tissues obtained from GLUT4-null mice as controls. Indirect immunohistochemistry, performed in human placentas, showed that intravillous stromal cells were conspicuously labeled by GLUT4 and revealed colocalization of GLUT4 transporters with insulin receptors. This study provides the first evidence that the insulinresponsive GLUT4 glucose transporter is present in human and rodent hemochorial placentas. Placental GLUT4 gene and protein levels were not modified in human pregnancy complicated by insulin-dependent diabetes mellitus. The significance of the high level of GLUT4 protein in human placenta remains to be elucidated, because, so far, this organ was not considered to be insulin-sensitive, with regard to glucose transport.

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