Unexpected Embryonic Stem (ES) Cell Mutations Represent a Concern in Gene Targeting: Lessons from "Fierce" Mice

Ravinesh A. Kumar, Ka Ling Chan, Ambrose H W Wong, Ken Q. Little, Evica Rajcan-Separovic, Brett S. Abrahams, Elizabeth M. Simpson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The exceptional value of gene targeting technology to generate mouse models of human disease exists under the shadow of potential genetic errors. We previously observed an unexpected brain-behavior phenotype that resulted from a gene-targeting experiment designed to delete the Zfa gene. Given that the transcription of Zfa is restricted to the germ cell lineage of adult testis, it was both a surprise and a concern when the resulting mice had a phenotype present in both sexes that included abnormal brains and violent behavior. We hypothesized that an unrelated mutation may have been responsible for the unexpected phenotype. Here we show that the single gene mutation, Nr2e1 frc (fierce), which was responsible for the brain-behavior phenotype, existed in the embryonic stem (ES) cell even before the derivation of the Zfa knockout mice. Our work thus highlights a concern in gene targeting, namely, that ES cells can harbor unexpected mutations, which can lead to genotype-phenotype misattribution. Based on our findings, we caution the gene-targeting community to use low-passage ES cells, to characterize mice derived from more than one independently targeted ES cell clone, and to backcross mice to allow for segregation of distant but linked mutations.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalGenesis
Volume38
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

Fingerprint

Gene Targeting
Embryonic Stem Cells
Phenotype
Mutation
Brain
Cell Lineage
Knockout Mice
Germ Cells
Genes
Testis
Clone Cells
Genotype
Technology

Keywords

  • Aggression
  • Brain development
  • E14TG2a
  • Homologous recombination
  • Mouse models
  • Nr2e1
  • Nuclear receptor
  • Tailless
  • Violence

ASJC Scopus subject areas

  • Genetics

Cite this

Kumar, R. A., Chan, K. L., Wong, A. H. W., Little, K. Q., Rajcan-Separovic, E., Abrahams, B. S., & Simpson, E. M. (2004). Unexpected Embryonic Stem (ES) Cell Mutations Represent a Concern in Gene Targeting: Lessons from "Fierce" Mice. Genesis, 38(2), 51-57. https://doi.org/10.1002/gene.20001

Unexpected Embryonic Stem (ES) Cell Mutations Represent a Concern in Gene Targeting : Lessons from "Fierce" Mice. / Kumar, Ravinesh A.; Chan, Ka Ling; Wong, Ambrose H W; Little, Ken Q.; Rajcan-Separovic, Evica; Abrahams, Brett S.; Simpson, Elizabeth M.

In: Genesis, Vol. 38, No. 2, 02.2004, p. 51-57.

Research output: Contribution to journalArticle

Kumar, RA, Chan, KL, Wong, AHW, Little, KQ, Rajcan-Separovic, E, Abrahams, BS & Simpson, EM 2004, 'Unexpected Embryonic Stem (ES) Cell Mutations Represent a Concern in Gene Targeting: Lessons from "Fierce" Mice', Genesis, vol. 38, no. 2, pp. 51-57. https://doi.org/10.1002/gene.20001
Kumar RA, Chan KL, Wong AHW, Little KQ, Rajcan-Separovic E, Abrahams BS et al. Unexpected Embryonic Stem (ES) Cell Mutations Represent a Concern in Gene Targeting: Lessons from "Fierce" Mice. Genesis. 2004 Feb;38(2):51-57. https://doi.org/10.1002/gene.20001
Kumar, Ravinesh A. ; Chan, Ka Ling ; Wong, Ambrose H W ; Little, Ken Q. ; Rajcan-Separovic, Evica ; Abrahams, Brett S. ; Simpson, Elizabeth M. / Unexpected Embryonic Stem (ES) Cell Mutations Represent a Concern in Gene Targeting : Lessons from "Fierce" Mice. In: Genesis. 2004 ; Vol. 38, No. 2. pp. 51-57.
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