Unequal sister chromatid exchange. A mechanism affecting Ig gene arrangement and expression

S. A. Tilley, B. K. Birshtein

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Two γ2a gene forms in the MPC11 mouse myeloma cell line (γ2b, κ) have been localized to the expressed H chain chromosome, where they exist in tandem downstream of the expressed γ2b gene. The γ2a gene duplication has apparently occurred by spontaneous, unequal sister chromatid exchange (SCE) in a B lymphoid precursor of MPC11 or in MPC11 itself. It is especially significant that either of the tandem γ2a gene forms may be used in the class switch from γ2b to γ2a production occurring in the MPC11 line, indicating that heavy chain constant region (C(H)) gene duplication (or deletion) may be a mechanism normally impinging on C(H) gene expression. The γ2a genes of MPC11 undergo further copy number variation via unequal SCE in variants of this line derived by mutagenesis and selection for altered H chain production. The remarkable frequency with which copy number variation occurs in these cells suggests that unequal SCE may play a physiological role in C(H) gene arrangement and expression.

Original languageEnglish (US)
Pages (from-to)675-694
Number of pages20
JournalJournal of Experimental Medicine
Volume162
Issue number2
StatePublished - 1985

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Immunoglobulin Genes
Sister Chromatid Exchange
Gene Order
Gene Expression
Gene Duplication
Genes
Gene Deletion
Mutagenesis
Chromosomes
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Unequal sister chromatid exchange. A mechanism affecting Ig gene arrangement and expression. / Tilley, S. A.; Birshtein, B. K.

In: Journal of Experimental Medicine, Vol. 162, No. 2, 1985, p. 675-694.

Research output: Contribution to journalArticle

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