Ultrastructural distribution of poly (A)+ RNA during Trypanosoma cruzi-cardiomyocyte interaction in vitro: A quantitative analysis of the total mRNA content by in situ hybridization

Mirian Claudia S Pereira, Robert H. Singer, Maria De Nazareth S L De Meirelles

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ultrastructural in situ hybridization was used to visualize the spatial distribution of poly (A)+ RNA and quantitate its relative amount within different cellular compartments of cardiomyocytes after T. cruzi infection. The amount of poly (A)+ RNA remained about the same up to 24 h post-infection. In contrast, its content was reduced 65% after 72 h of interaction, showing a marked decrease in the cell cytoplasm. This decline in poly (A)+ RNA level in host cell cytoplasm was concomitant with intracellular proliferation of T. cruzi amastigotes. Thus, T. cruzi may affect host cell cytoplasmic mRNA stability, associated with the parasite's intracellular multiplication.

Original languageEnglish (US)
Pages (from-to)264-270
Number of pages7
JournalJournal of Eukaryotic Microbiology
Volume47
Issue number3
StatePublished - 2000

Fingerprint

Trypanosoma cruzi
messenger RNA
Cardiac Myocytes
in situ hybridization
In Situ Hybridization
quantitative analysis
Messenger RNA
Cytoplasm
cytoplasm
amastigotes
RNA Stability
cells
Infection
infection
Parasites
spatial distribution
parasites
cardiomyocytes
In Vitro Techniques

Keywords

  • Chagas' disease
  • Heart muscle cells
  • Hemoflagellate
  • Ultrastructural in situ hybridization

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Cite this

Ultrastructural distribution of poly (A)+ RNA during Trypanosoma cruzi-cardiomyocyte interaction in vitro : A quantitative analysis of the total mRNA content by in situ hybridization. / Pereira, Mirian Claudia S; Singer, Robert H.; De Meirelles, Maria De Nazareth S L.

In: Journal of Eukaryotic Microbiology, Vol. 47, No. 3, 2000, p. 264-270.

Research output: Contribution to journalArticle

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