Tyr682 in the intracellular domain of APP regulates amyloidogenic APP processing in vivo

Alessia P M Barbagallo, Richard Weldon, Robert Tamayev, Dawang Zhou, Luca Giliberto, Oded Foreman, Luciano D'Adamio

Research output: Contribution to journalArticle

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Abstract

Background: The pathogenesis of Alzheimer's disease is attributed to misfolding of Amyloid-β (Aβ) peptides. Aβ is generated during amyloidogenic processing of Aβ-precursor protein (APP). Another characteristic of the AD brain is increased phosphorylation of APP amino acid Tyr682. Tyr682 is part of the Y682ENPTY687 motif, a docking site for interaction with cytosolic proteins that regulate APP metabolism and signaling. For example, normal Aβ generation and secretion are dependent upon Tyr682 in vitro. However, physiological functions of Tyr682 are unknown. Methodology/Principal Findings: To this end, we have generated an APP Y682G knock-in (KI) mouse to help dissect the role of APP Tyr682in vivo. We have analyzed proteolytic products from both the amyloidogenic and non-amyloidogenic processing of APP and measure a profound shift towards non-amyloidogenic processing in APP KI mice. In addition, we demonstrate the essential nature of amino acid Tyr682 for the APP/Fe65 interaction in vivo. Conclusions/Significance: Together, these observations point to an essential role of APP intracellular domain for normal APP processing and function in vivo, and provide rationale for further studies into physiological functions associated with this important phosphorylation site.

Original languageEnglish (US)
Article numbere15503
JournalPLoS One
Volume5
Issue number11
DOIs
StatePublished - 2010

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Amyloidogenic Proteins
Protein Precursors
Processing
proteins
Phosphorylation
phosphorylation
Essential Amino Acids
amino acids
mice
Amyloid
amyloid
Alzheimer disease
protein metabolism
Metabolism
Alzheimer Disease
Brain
pathogenesis
Amino Acids
secretion
peptides

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Barbagallo, A. P. M., Weldon, R., Tamayev, R., Zhou, D., Giliberto, L., Foreman, O., & D'Adamio, L. (2010). Tyr682 in the intracellular domain of APP regulates amyloidogenic APP processing in vivo. PLoS One, 5(11), [e15503]. https://doi.org/10.1371/journal.pone.0015503

Tyr682 in the intracellular domain of APP regulates amyloidogenic APP processing in vivo. / Barbagallo, Alessia P M; Weldon, Richard; Tamayev, Robert; Zhou, Dawang; Giliberto, Luca; Foreman, Oded; D'Adamio, Luciano.

In: PLoS One, Vol. 5, No. 11, e15503, 2010.

Research output: Contribution to journalArticle

Barbagallo, APM, Weldon, R, Tamayev, R, Zhou, D, Giliberto, L, Foreman, O & D'Adamio, L 2010, 'Tyr682 in the intracellular domain of APP regulates amyloidogenic APP processing in vivo', PLoS One, vol. 5, no. 11, e15503. https://doi.org/10.1371/journal.pone.0015503
Barbagallo APM, Weldon R, Tamayev R, Zhou D, Giliberto L, Foreman O et al. Tyr682 in the intracellular domain of APP regulates amyloidogenic APP processing in vivo. PLoS One. 2010;5(11). e15503. https://doi.org/10.1371/journal.pone.0015503
Barbagallo, Alessia P M ; Weldon, Richard ; Tamayev, Robert ; Zhou, Dawang ; Giliberto, Luca ; Foreman, Oded ; D'Adamio, Luciano. / Tyr682 in the intracellular domain of APP regulates amyloidogenic APP processing in vivo. In: PLoS One. 2010 ; Vol. 5, No. 11.
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