Two types of phosphofructokinase-1 differentially regulate the glycolytic pathway in insulin-stimulated chicken skeletal muscle

Yoshinori Seki, Kan Sato, Tatsuyoshi Kono, Yukio Akiba

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To elucidate the precise regulation of glucose homeostasis in chicken skeletal muscle, expression of muscle- and liver-type phosphofructokinase-1 (EC:2.7.1.11, PFK-M, PFK-L) was characterized in the insulin-stimulated state by Real-Time PCR. Firstly, chicken PFK-M and PFK-L full-length cDNA sequences were identified. The deduced amino acid sequences were 81.6% and 86.5% identical with human PFK-M and PFK-L, respectively. In pectoralis superficialis (PS) muscle and extensor digitorum longus (EDL), PFK-M mRNA levels were unchanged following insulin stimulation. Surprisingly, although mammalian PFK-L has been reported to be expressed in liver, kidney and brain, chicken PFK-L was not detected in liver and kidney, however, strong expression was detected in skeletal muscle and brain by Northern blot analysis. However, using PCR, PFK-L mRNA was detected in liver. Taken together, chicken PFK-L mRNA expression was at a very low level, below the detection limit of Northern blot analysis. Chicken PFK-L mRNA levels were increased 200% in PS muscle but decreased by 40% in EDL following insulin stimulation. These results suggest that two types of PFK regulate the glycolytic pathway in the insulin-stimulated state and, therefore, that glucose metabolism in chicken skeletal muscle may be regulated in a very different manner compared to mammals.

Original languageEnglish (US)
Pages (from-to)344-350
Number of pages7
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume143
Issue number3
DOIs
StatePublished - Mar 2006
Externally publishedYes

Fingerprint

Phosphofructokinase-1
Muscle
Chickens
Skeletal Muscle
Insulin
Liver
Pectoralis Muscles
Messenger RNA
Muscle Type Phosphofructokinase-1
Liver Type Phosphofructokinase-1
Northern Blotting
Brain
Kidney
Glucose
Mammals
Metabolism
Limit of Detection
Real-Time Polymerase Chain Reaction
Amino Acid Sequence
Homeostasis

Keywords

  • Chicken
  • Glucose metabolism
  • Insulin
  • Molecular cloning
  • mRNA expression
  • Phosphofructokinase-1
  • Real-Time PCR
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this

Two types of phosphofructokinase-1 differentially regulate the glycolytic pathway in insulin-stimulated chicken skeletal muscle. / Seki, Yoshinori; Sato, Kan; Kono, Tatsuyoshi; Akiba, Yukio.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 143, No. 3, 03.2006, p. 344-350.

Research output: Contribution to journalArticle

@article{44570d5864084f579df9c9cea9568022,
title = "Two types of phosphofructokinase-1 differentially regulate the glycolytic pathway in insulin-stimulated chicken skeletal muscle",
abstract = "To elucidate the precise regulation of glucose homeostasis in chicken skeletal muscle, expression of muscle- and liver-type phosphofructokinase-1 (EC:2.7.1.11, PFK-M, PFK-L) was characterized in the insulin-stimulated state by Real-Time PCR. Firstly, chicken PFK-M and PFK-L full-length cDNA sequences were identified. The deduced amino acid sequences were 81.6{\%} and 86.5{\%} identical with human PFK-M and PFK-L, respectively. In pectoralis superficialis (PS) muscle and extensor digitorum longus (EDL), PFK-M mRNA levels were unchanged following insulin stimulation. Surprisingly, although mammalian PFK-L has been reported to be expressed in liver, kidney and brain, chicken PFK-L was not detected in liver and kidney, however, strong expression was detected in skeletal muscle and brain by Northern blot analysis. However, using PCR, PFK-L mRNA was detected in liver. Taken together, chicken PFK-L mRNA expression was at a very low level, below the detection limit of Northern blot analysis. Chicken PFK-L mRNA levels were increased 200{\%} in PS muscle but decreased by 40{\%} in EDL following insulin stimulation. These results suggest that two types of PFK regulate the glycolytic pathway in the insulin-stimulated state and, therefore, that glucose metabolism in chicken skeletal muscle may be regulated in a very different manner compared to mammals.",
keywords = "Chicken, Glucose metabolism, Insulin, Molecular cloning, mRNA expression, Phosphofructokinase-1, Real-Time PCR, Skeletal muscle",
author = "Yoshinori Seki and Kan Sato and Tatsuyoshi Kono and Yukio Akiba",
year = "2006",
month = "3",
doi = "10.1016/j.cbpb.2005.12.006",
language = "English (US)",
volume = "143",
pages = "344--350",
journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",
issn = "1096-4959",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Two types of phosphofructokinase-1 differentially regulate the glycolytic pathway in insulin-stimulated chicken skeletal muscle

AU - Seki, Yoshinori

AU - Sato, Kan

AU - Kono, Tatsuyoshi

AU - Akiba, Yukio

PY - 2006/3

Y1 - 2006/3

N2 - To elucidate the precise regulation of glucose homeostasis in chicken skeletal muscle, expression of muscle- and liver-type phosphofructokinase-1 (EC:2.7.1.11, PFK-M, PFK-L) was characterized in the insulin-stimulated state by Real-Time PCR. Firstly, chicken PFK-M and PFK-L full-length cDNA sequences were identified. The deduced amino acid sequences were 81.6% and 86.5% identical with human PFK-M and PFK-L, respectively. In pectoralis superficialis (PS) muscle and extensor digitorum longus (EDL), PFK-M mRNA levels were unchanged following insulin stimulation. Surprisingly, although mammalian PFK-L has been reported to be expressed in liver, kidney and brain, chicken PFK-L was not detected in liver and kidney, however, strong expression was detected in skeletal muscle and brain by Northern blot analysis. However, using PCR, PFK-L mRNA was detected in liver. Taken together, chicken PFK-L mRNA expression was at a very low level, below the detection limit of Northern blot analysis. Chicken PFK-L mRNA levels were increased 200% in PS muscle but decreased by 40% in EDL following insulin stimulation. These results suggest that two types of PFK regulate the glycolytic pathway in the insulin-stimulated state and, therefore, that glucose metabolism in chicken skeletal muscle may be regulated in a very different manner compared to mammals.

AB - To elucidate the precise regulation of glucose homeostasis in chicken skeletal muscle, expression of muscle- and liver-type phosphofructokinase-1 (EC:2.7.1.11, PFK-M, PFK-L) was characterized in the insulin-stimulated state by Real-Time PCR. Firstly, chicken PFK-M and PFK-L full-length cDNA sequences were identified. The deduced amino acid sequences were 81.6% and 86.5% identical with human PFK-M and PFK-L, respectively. In pectoralis superficialis (PS) muscle and extensor digitorum longus (EDL), PFK-M mRNA levels were unchanged following insulin stimulation. Surprisingly, although mammalian PFK-L has been reported to be expressed in liver, kidney and brain, chicken PFK-L was not detected in liver and kidney, however, strong expression was detected in skeletal muscle and brain by Northern blot analysis. However, using PCR, PFK-L mRNA was detected in liver. Taken together, chicken PFK-L mRNA expression was at a very low level, below the detection limit of Northern blot analysis. Chicken PFK-L mRNA levels were increased 200% in PS muscle but decreased by 40% in EDL following insulin stimulation. These results suggest that two types of PFK regulate the glycolytic pathway in the insulin-stimulated state and, therefore, that glucose metabolism in chicken skeletal muscle may be regulated in a very different manner compared to mammals.

KW - Chicken

KW - Glucose metabolism

KW - Insulin

KW - Molecular cloning

KW - mRNA expression

KW - Phosphofructokinase-1

KW - Real-Time PCR

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=33344475508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33344475508&partnerID=8YFLogxK

U2 - 10.1016/j.cbpb.2005.12.006

DO - 10.1016/j.cbpb.2005.12.006

M3 - Article

VL - 143

SP - 344

EP - 350

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

SN - 1096-4959

IS - 3

ER -