Two ribosome binding sites from the gene 0.3 messenger RNA of bacteriophage T7

Joan Argetsinger Steitz, Ruth A. Bryan

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Ribosome-protected regions have been isolated and analyzed from the bacteriophage T7 gene 0.3 mRNA labeled in vivo. Two discrete sites which are nearly equally protected by ribosomes are obtained from what was previously assumed to be a monocistronic message. Use of appropriate T7 deletion mutant RNAs has allowed mapping of both ribosome-recognized regions. Site a is positioned very close to the 5′ terminus of the mRNA and is apparently the initiator region for the major gene 0.3 protein, which acts to overcome the host DNA restriction system. Site b is located within several hundred nucleotides of the 3′ end of the RNA and probably initiates synthesis of a small polypeptide of unknown function. Both ribosome binding sites exhibit features common to other initiator regions from Escherichia coli and bacteriophage mRNAs. The proximity of site a to the RNase III cleavage site at the left end of gene 0.3 may explain why processing by RNase III is required for efficient translation of the major gene 0.3 protein.

Original languageEnglish (US)
Pages (from-to)527-543
Number of pages17
JournalJournal of Molecular Biology
Volume114
Issue number4
DOIs
StatePublished - Aug 25 1977
Externally publishedYes

Fingerprint

RNA Phages
Bacteriophage T7
Ribosomes
Binding Sites
Ribonuclease III
Messenger RNA
Genes
RNA
Bacteriophages
Proteins
Nucleotides
Escherichia coli
Peptides
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Two ribosome binding sites from the gene 0.3 messenger RNA of bacteriophage T7. / Steitz, Joan Argetsinger; Bryan, Ruth A.

In: Journal of Molecular Biology, Vol. 114, No. 4, 25.08.1977, p. 527-543.

Research output: Contribution to journalArticle

Steitz, Joan Argetsinger ; Bryan, Ruth A. / Two ribosome binding sites from the gene 0.3 messenger RNA of bacteriophage T7. In: Journal of Molecular Biology. 1977 ; Vol. 114, No. 4. pp. 527-543.
@article{ead36597cdd64151846d017cf104e689,
title = "Two ribosome binding sites from the gene 0.3 messenger RNA of bacteriophage T7",
abstract = "Ribosome-protected regions have been isolated and analyzed from the bacteriophage T7 gene 0.3 mRNA labeled in vivo. Two discrete sites which are nearly equally protected by ribosomes are obtained from what was previously assumed to be a monocistronic message. Use of appropriate T7 deletion mutant RNAs has allowed mapping of both ribosome-recognized regions. Site a is positioned very close to the 5′ terminus of the mRNA and is apparently the initiator region for the major gene 0.3 protein, which acts to overcome the host DNA restriction system. Site b is located within several hundred nucleotides of the 3′ end of the RNA and probably initiates synthesis of a small polypeptide of unknown function. Both ribosome binding sites exhibit features common to other initiator regions from Escherichia coli and bacteriophage mRNAs. The proximity of site a to the RNase III cleavage site at the left end of gene 0.3 may explain why processing by RNase III is required for efficient translation of the major gene 0.3 protein.",
author = "Steitz, {Joan Argetsinger} and Bryan, {Ruth A.}",
year = "1977",
month = "8",
day = "25",
doi = "10.1016/0022-2836(77)90176-0",
language = "English (US)",
volume = "114",
pages = "527--543",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Two ribosome binding sites from the gene 0.3 messenger RNA of bacteriophage T7

AU - Steitz, Joan Argetsinger

AU - Bryan, Ruth A.

PY - 1977/8/25

Y1 - 1977/8/25

N2 - Ribosome-protected regions have been isolated and analyzed from the bacteriophage T7 gene 0.3 mRNA labeled in vivo. Two discrete sites which are nearly equally protected by ribosomes are obtained from what was previously assumed to be a monocistronic message. Use of appropriate T7 deletion mutant RNAs has allowed mapping of both ribosome-recognized regions. Site a is positioned very close to the 5′ terminus of the mRNA and is apparently the initiator region for the major gene 0.3 protein, which acts to overcome the host DNA restriction system. Site b is located within several hundred nucleotides of the 3′ end of the RNA and probably initiates synthesis of a small polypeptide of unknown function. Both ribosome binding sites exhibit features common to other initiator regions from Escherichia coli and bacteriophage mRNAs. The proximity of site a to the RNase III cleavage site at the left end of gene 0.3 may explain why processing by RNase III is required for efficient translation of the major gene 0.3 protein.

AB - Ribosome-protected regions have been isolated and analyzed from the bacteriophage T7 gene 0.3 mRNA labeled in vivo. Two discrete sites which are nearly equally protected by ribosomes are obtained from what was previously assumed to be a monocistronic message. Use of appropriate T7 deletion mutant RNAs has allowed mapping of both ribosome-recognized regions. Site a is positioned very close to the 5′ terminus of the mRNA and is apparently the initiator region for the major gene 0.3 protein, which acts to overcome the host DNA restriction system. Site b is located within several hundred nucleotides of the 3′ end of the RNA and probably initiates synthesis of a small polypeptide of unknown function. Both ribosome binding sites exhibit features common to other initiator regions from Escherichia coli and bacteriophage mRNAs. The proximity of site a to the RNase III cleavage site at the left end of gene 0.3 may explain why processing by RNase III is required for efficient translation of the major gene 0.3 protein.

UR - http://www.scopus.com/inward/record.url?scp=0017408308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017408308&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(77)90176-0

DO - 10.1016/0022-2836(77)90176-0

M3 - Article

C2 - 915941

AN - SCOPUS:0017408308

VL - 114

SP - 527

EP - 543

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -