Two non-vesicular ATP release pathways in the mouse erythrocyte membrane

Feng Qiu, Junjie Wang, David C. Spray, Eliana Scemes, Gerhard Dahl

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here we show that erythrocytes of Panx1 -/- mice lend further support to this conclusion. However, ATP release, although attenuated, was still observed in Panx1 -/- mouse erythrocytes. In contrast to Panx1 +/+ cells, this release was not correlated with uptake of extracellularly applied dyes, was insensitive to Panx1 channel blockers, and was inhibited by dipyridamole and stimulated by iloprost. Thus, in erythrocytes, two independent pathways mediate the release of ATP. We also show that glyburide is a strong inhibitor of Panx1 channels.

Original languageEnglish (US)
Pages (from-to)3430-3435
Number of pages6
JournalFEBS Letters
Volume585
Issue number21
DOIs
StatePublished - Nov 4 2011

Fingerprint

Erythrocyte Membrane
Adenosine Triphosphate
Erythrocytes
Membranes
Iloprost
Dipyridamole
Glyburide
Permeation
Coloring Agents
Anura

Keywords

  • ATP
  • Dipyridamole
  • Erythrocyte
  • Glyburide
  • Pannexin
  • Probenecid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Two non-vesicular ATP release pathways in the mouse erythrocyte membrane. / Qiu, Feng; Wang, Junjie; Spray, David C.; Scemes, Eliana; Dahl, Gerhard.

In: FEBS Letters, Vol. 585, No. 21, 04.11.2011, p. 3430-3435.

Research output: Contribution to journalArticle

Qiu, Feng ; Wang, Junjie ; Spray, David C. ; Scemes, Eliana ; Dahl, Gerhard. / Two non-vesicular ATP release pathways in the mouse erythrocyte membrane. In: FEBS Letters. 2011 ; Vol. 585, No. 21. pp. 3430-3435.
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