Two non-vesicular ATP release pathways in the mouse erythrocyte membrane

Feng Qiu, Junjie Wang, David C. Spray, Eliana Scemes, Gerhard Dahl

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here we show that erythrocytes of Panx1 -/- mice lend further support to this conclusion. However, ATP release, although attenuated, was still observed in Panx1 -/- mouse erythrocytes. In contrast to Panx1 +/+ cells, this release was not correlated with uptake of extracellularly applied dyes, was insensitive to Panx1 channel blockers, and was inhibited by dipyridamole and stimulated by iloprost. Thus, in erythrocytes, two independent pathways mediate the release of ATP. We also show that glyburide is a strong inhibitor of Panx1 channels.

Original languageEnglish (US)
Pages (from-to)3430-3435
Number of pages6
JournalFEBS Letters
Volume585
Issue number21
DOIs
StatePublished - Nov 4 2011

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Keywords

  • ATP
  • Dipyridamole
  • Erythrocyte
  • Glyburide
  • Pannexin
  • Probenecid

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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