Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase

Gregory C. Rogers, Stephen L. Rogers, Tamara A. Schwimmer, Stephanie C. Ems-McClung, Claire E. Walczak, Ronald D. Vale, Jonathan M. Scholey, David J. Sharp

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

During anaphase identical sister chromatids separate and move towards opposite poles of the mitotic spindle. In the spindle, kinetochore microtubules have their plus ends embedded in the kinetochore and their minus ends at the spindle pole. Two models have been proposed to account for the movement of chromatids during anaphase. In the 'Pac-Man' model, kinetochores induce the depolymerization of kinetochore microtubules at their plus ends, which allows chromatids to move towards the pole by 'chewing up' microtubule tracks. In the 'poleward flux' model, kinetochores anchor kinetochore microtubules and chromatids are pulled towards the poles through the depolymerization of kinetochore microtubules at the minus ends. Here, we show that two functionally distinct microtubule-destabilizing KinI kinesin enzymes (so named because they possess a kinesin-like ATPase domain positioned internally within the polypeptide) are responsible for normal chromatid-to-pole motion in Drosophila. One of them, KLP59C, is required to depolymerize kinetochore microtubules at their kinetochore-associated plus ends, thereby contributing to chromatid motility through a Pac-Man-based mechanism. The other, KLP10A, is required to depolymerize microtubules at their pole-associated minus ends, thereby moving chromatids by means of poleward flux.

Original languageEnglish (US)
Pages (from-to)364-370
Number of pages7
JournalNature
Volume427
Issue number6972
DOIs
StatePublished - Jan 22 2004

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Kinetochores
Kinesin
Anaphase
Chromatids
Siblings
Microtubules
Spindle Poles
Drive
Spindle Apparatus
Mastication
Drosophila
Adenosine Triphosphatases
Peptides

ASJC Scopus subject areas

  • General

Cite this

Rogers, G. C., Rogers, S. L., Schwimmer, T. A., Ems-McClung, S. C., Walczak, C. E., Vale, R. D., ... Sharp, D. J. (2004). Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature, 427(6972), 364-370. https://doi.org/10.1038/nature02256

Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. / Rogers, Gregory C.; Rogers, Stephen L.; Schwimmer, Tamara A.; Ems-McClung, Stephanie C.; Walczak, Claire E.; Vale, Ronald D.; Scholey, Jonathan M.; Sharp, David J.

In: Nature, Vol. 427, No. 6972, 22.01.2004, p. 364-370.

Research output: Contribution to journalArticle

Rogers, GC, Rogers, SL, Schwimmer, TA, Ems-McClung, SC, Walczak, CE, Vale, RD, Scholey, JM & Sharp, DJ 2004, 'Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase', Nature, vol. 427, no. 6972, pp. 364-370. https://doi.org/10.1038/nature02256
Rogers GC, Rogers SL, Schwimmer TA, Ems-McClung SC, Walczak CE, Vale RD et al. Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature. 2004 Jan 22;427(6972):364-370. https://doi.org/10.1038/nature02256
Rogers, Gregory C. ; Rogers, Stephen L. ; Schwimmer, Tamara A. ; Ems-McClung, Stephanie C. ; Walczak, Claire E. ; Vale, Ronald D. ; Scholey, Jonathan M. ; Sharp, David J. / Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. In: Nature. 2004 ; Vol. 427, No. 6972. pp. 364-370.
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