Two functional S100A4 monomers are necessary for regulating nonmuscle myosin-IIA and HCT116 cell invasion

Reniqua P. House, Maria Pozzuto, Purvi Patel, Natalia Dulianinova, Zhong Hua Li, Wendy D. Zencheck, Michele I. Vitolo, David J. Weber, Anne R. Bresnick

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

S100A4, a member of the Ca 2+-activated S100 protein family, regulates the motility and invasiveness of cancer cells. Moreover, high S100A4 expression levels correlate with poor patient survival in several cancers. Although biochemical, biophysical, and structural data indicate that S100A4 is a noncovalent dimer, it is unknown if two functional S100A4 monomers are required for the productive recognition of protein targets and the promotion of cell invasion. To address this question, we created covalently linked S100A4 dimers using a glycine rich flexible linker. The single-chain S100A4 (sc-S100A4) proteins exhibited wild-type affinities for calcium and nonmuscle myosin-IIA, retained the ability to regulate nonmuscle myosin-IIA assembly, and promoted tumor cell invasion when expressed in S100A4-deficient colon carcinoma cells. Mutation of the two calcium-binding EF-hands in one monomer, while leaving the other monomer intact, caused a 30-60-fold reduction in binding affinity for nonmuscle myosin-IIA concomitant with a weakened ability to regulate the monomer-polymer equilibrium of nonmuscle myosin-IIA. Moreover, sc-S100A4 proteins with one monomer deficient in calcium responsiveness did not support S100A4-mediated colon carcinoma cell invasion. Cross-linking and titration data indicate that the S100A4 dimer binds a single myosin-IIA target peptide. These data are consistent with a model in which a single peptide forms interactions in the vicinity of the canonical target binding cleft of each monomer in such a manner that both target binding sites are required for the efficient interaction with myosin-IIA.

Original languageEnglish (US)
Pages (from-to)6920-6932
Number of pages13
JournalBiochemistry
Volume50
Issue number32
DOIs
StatePublished - Aug 16 2011

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Nonmuscle Myosin Type IIA
HCT116 Cells
Monomers
Cells
Dimers
Calcium
Colon
EF Hand Motifs
Carcinoma
Neoplasms
Peptides
Proteins
S100 Proteins
Titration
Glycine
Tumors
Polymers
Binding Sites
Mutation
Survival

ASJC Scopus subject areas

  • Biochemistry

Cite this

Two functional S100A4 monomers are necessary for regulating nonmuscle myosin-IIA and HCT116 cell invasion. / House, Reniqua P.; Pozzuto, Maria; Patel, Purvi; Dulianinova, Natalia; Li, Zhong Hua; Zencheck, Wendy D.; Vitolo, Michele I.; Weber, David J.; Bresnick, Anne R.

In: Biochemistry, Vol. 50, No. 32, 16.08.2011, p. 6920-6932.

Research output: Contribution to journalArticle

House, RP, Pozzuto, M, Patel, P, Dulianinova, N, Li, ZH, Zencheck, WD, Vitolo, MI, Weber, DJ & Bresnick, AR 2011, 'Two functional S100A4 monomers are necessary for regulating nonmuscle myosin-IIA and HCT116 cell invasion', Biochemistry, vol. 50, no. 32, pp. 6920-6932. https://doi.org/10.1021/bi200498q
House, Reniqua P. ; Pozzuto, Maria ; Patel, Purvi ; Dulianinova, Natalia ; Li, Zhong Hua ; Zencheck, Wendy D. ; Vitolo, Michele I. ; Weber, David J. ; Bresnick, Anne R. / Two functional S100A4 monomers are necessary for regulating nonmuscle myosin-IIA and HCT116 cell invasion. In: Biochemistry. 2011 ; Vol. 50, No. 32. pp. 6920-6932.
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AU - Patel, Purvi

AU - Dulianinova, Natalia

AU - Li, Zhong Hua

AU - Zencheck, Wendy D.

AU - Vitolo, Michele I.

AU - Weber, David J.

AU - Bresnick, Anne R.

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