Two-dimensional motility of a macrophage cell line on microcontact-printed fibronectin

Laurel E. Hind, Joanna L. Mackay, Dianne Cox, Daniel A. Hammer

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The ability of macrophages to migrate to sites of infection and inflammation is critical for their role in the innate immune response. Macrophage cell lines have made it possible to study the roles of individual proteins responsible for migration using molecular biology, but it has not been possible to reliably elicit the motility of macrophage cell lines in two dimensions. In the past, measurements of the motility of macrophage cell lines have been largely limited to transwell assays which provide limited quantitative information on motility and limited ability to visualize cell morphology. We used microcontact printing to create polydimethylsiloxane (PDMS) surfaces functionalized with fibronectin that otherwise support little macrophage adhesion. We used these surfaces to measure macrophage migration in two dimensions and found that these cells migrate efficiently in a uniform field of colony-stimulating factor-1, CSF-1. Knockdown of Cdc42 led to a nonstatistically significant reduction in motility, whereas chemical inhibition of PI3K activity led to a complete loss of motility. Inhibition of the RhoA kinase, ROCK, did not abolish the motility of these cells but caused a quantitative change in motility, reducing motility significantly on high concentrations of fibronectin but not on low concentrations. This study illustrates the importance of studying cell motility on well controlled materials to better understand the exact roles of specific proteins on cell migration.

Original languageEnglish (US)
Pages (from-to)542-554
Number of pages13
JournalCytoskeleton
Volume71
Issue number9
DOIs
StatePublished - Sep 1 2014

Fingerprint

Fibronectins
Macrophages
Cell Line
Cell Movement
Macrophage Colony-Stimulating Factor
Printing
Phosphatidylinositol 3-Kinases
Innate Immunity
Molecular Biology
Proteins
Phosphotransferases
Inflammation
Infection

Keywords

  • Fibronectin
  • Macrophage
  • Microcontact printing
  • Motility
  • Podosome

ASJC Scopus subject areas

  • Cell Biology
  • Structural Biology

Cite this

Two-dimensional motility of a macrophage cell line on microcontact-printed fibronectin. / Hind, Laurel E.; Mackay, Joanna L.; Cox, Dianne; Hammer, Daniel A.

In: Cytoskeleton, Vol. 71, No. 9, 01.09.2014, p. 542-554.

Research output: Contribution to journalArticle

Hind, Laurel E. ; Mackay, Joanna L. ; Cox, Dianne ; Hammer, Daniel A. / Two-dimensional motility of a macrophage cell line on microcontact-printed fibronectin. In: Cytoskeleton. 2014 ; Vol. 71, No. 9. pp. 542-554.
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