Two-dimensional DNA typing - A cost-effective way of analyzing complex mixtures of DNA fragments for sequence variations

Research output: Contribution to journalReview article

6 Scopus citations

Abstract

By two-dimensional (2D) DNA typing, multiple DNA fragments can be analyzed in parallel for all possible sequence variations. The method involves electrophoretic separation in polyacrylamide gels, first by size and subsequently on the basis of basepair sequence in a denaturing gradient. The system has been automated partly and a dedicated image analysis system for rapid interpretation of the often complex spot patterns was developed. In this review, the basic principles of 2D DNA typing, its methodology, and some major applications in genetic studies of animals, plants, and microorganisms will be discussed, with the emphasis on human genetics.

Original languageEnglish (US)
Pages (from-to)275-295
Number of pages21
JournalMolecular Biotechnology
Volume4
Issue number3
DOIs
StatePublished - Dec 1 1995
Externally publishedYes

Keywords

  • DNA sequence variation
  • automatic two-dimensional DNA electrophoresis
  • denaturing gradient gel electrophoresis
  • gene scanning
  • genome scanning
  • mutations

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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