Abstract
DNA typing based on gel electrophoretic separation of DNA fragments, followed by hybridization analysis, has become an important analytical tool in areas ranging from forensic science to population biology. This approach can be extended by combining size separation with sequence-specific separation in denaturing gradient gels; this creates a high resolution two-dimensional pattern. The high information content of this system means that very closely related individuals (even monozygotic twins) can be distinguished and that the genetic events associated with development or cancer, for instance, can be followed. Ultimately, 2-D DNA typing could lead to computerized matching of a single individual's genome to a database of genetic markers.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 336-341 |
| Number of pages | 6 |
| Journal | Trends in Biotechnology |
| Volume | 7 |
| Issue number | 12 |
| DOIs | |
| State | Published - 1989 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Bioengineering
Cite this
Two-dimensional DNA typing. / Uitterlinden, AndréG; Vijg, Jan.
In: Trends in Biotechnology, Vol. 7, No. 12, 1989, p. 336-341.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two-dimensional DNA typing
AU - Uitterlinden, AndréG
AU - Vijg, Jan
PY - 1989
Y1 - 1989
N2 - DNA typing based on gel electrophoretic separation of DNA fragments, followed by hybridization analysis, has become an important analytical tool in areas ranging from forensic science to population biology. This approach can be extended by combining size separation with sequence-specific separation in denaturing gradient gels; this creates a high resolution two-dimensional pattern. The high information content of this system means that very closely related individuals (even monozygotic twins) can be distinguished and that the genetic events associated with development or cancer, for instance, can be followed. Ultimately, 2-D DNA typing could lead to computerized matching of a single individual's genome to a database of genetic markers.
AB - DNA typing based on gel electrophoretic separation of DNA fragments, followed by hybridization analysis, has become an important analytical tool in areas ranging from forensic science to population biology. This approach can be extended by combining size separation with sequence-specific separation in denaturing gradient gels; this creates a high resolution two-dimensional pattern. The high information content of this system means that very closely related individuals (even monozygotic twins) can be distinguished and that the genetic events associated with development or cancer, for instance, can be followed. Ultimately, 2-D DNA typing could lead to computerized matching of a single individual's genome to a database of genetic markers.
UR - http://www.scopus.com/inward/record.url?scp=0024813399&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024813399&partnerID=8YFLogxK
U2 - 10.1016/0167-7799(89)90033-4
DO - 10.1016/0167-7799(89)90033-4
M3 - Article
AN - SCOPUS:0024813399
VL - 7
SP - 336
EP - 341
JO - Trends in Biotechnology
JF - Trends in Biotechnology
SN - 0167-7799
IS - 12
ER -