Two-dimensional DNA typing

AndréG G. Uitterlinden, Jan Vijg

Research output: Contribution to journalReview article

9 Scopus citations

Abstract

DNA typing based on gel electrophoretic separation of DNA fragments, followed by hybridization analysis, has become an important analytical tool in areas ranging from forensic science to population biology. This approach can be extended by combining size separation with sequence-specific separation in denaturing gradient gels; this creates a high resolution two-dimensional pattern. The high information content of this system means that very closely related individuals (even monozygotic twins) can be distinguished and that the genetic events associated with development or cancer, for instance, can be followed. Ultimately, 2-D DNA typing could lead to computerized matching of a single individual's genome to a database of genetic markers.

Original languageEnglish (US)
Pages (from-to)336-341
Number of pages6
JournalTrends in Biotechnology
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 1989

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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