Two-dimensional DNA fingerprinting of human individuals

A. G. Uitterlinden, P. E. Slagboom, D. L. Knook, Jan Vijg

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The limiting factor in the presently available techniques for the detection of DNA sequence variation in the human genome is the low resolution of Southern blot analysis. To increase the analytical power of this technique, we applied size fractionation of genomic DNA restriction fragments in conjunction with their sequence-dependent separation in denaturing gradient gels; the two-dimensional separation patterns obtained were subsequently transferred to nylon membranes. Hybridization analysis using minisatellite core sequences as probes resulted in two-dimensional genomic DNA fingerprints with a resolution of up to 625 separated spots per probe per human individual; by conventional Southern blot analysis, only 20-30 bands can be resolved. Using the two-dimensional DNA fingerprinting technique, we demonstrate in a small human pedigree the simultaneous transmission of 37 polymorphic fragments (out of 365 spots) for probe 33.15 and 105 polymorphic fragments (out of 625 spots) for probe 33.6. In addition, a mutation was detected in this pedigree by probe 33.6. We anticipate that this method will be of great use in studies aimed at (i) measuring human mutation frequencies, (ii) associating genetic variation with disease, (iii) analyzing genomic instability in relation to cancer and aging, and (iv) linkage analysis and mapping of disease genes.

Original languageEnglish (US)
Pages (from-to)2742-2746
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number8
StatePublished - 1989
Externally publishedYes

Fingerprint

DNA Fingerprinting
Pedigree
Southern Blotting
Minisatellite Repeats
Genomic Instability
Chromosome Mapping
Nylons
Human Genome
Mutation Rate
Gels
Mutation
Membranes
DNA
Genes
Neoplasms

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Two-dimensional DNA fingerprinting of human individuals. / Uitterlinden, A. G.; Slagboom, P. E.; Knook, D. L.; Vijg, Jan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 8, 1989, p. 2742-2746.

Research output: Contribution to journalArticle

Uitterlinden, A. G. ; Slagboom, P. E. ; Knook, D. L. ; Vijg, Jan. / Two-dimensional DNA fingerprinting of human individuals. In: Proceedings of the National Academy of Sciences of the United States of America. 1989 ; Vol. 86, No. 8. pp. 2742-2746.
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