Two-dimensional DNA electrophoresis: state of the art and applications

Vincenzo Romano-Spica, Jan Vijg

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Abstract

Abstract: Two-dimensional DNA electrophoresis allows the analysis of multiple DNA fragments based on both their size and sequence properties. The often complex gel separation patterns represent information-rich data sets that may be analyzed with dedicated image analysis systems. The introduction of a denaturing gradient in the second dimension enhances the capability of the method to identify sequence variations, and the recent construction of automated instruments opens up new ways for the diffusion of the procedure into the laboratory routine. In this review, the state of the art of the technique, its general principles and basic methodology will be discussed together with major applications in different fields, including environmental microbiology, mutation detection and human genetics.

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