Abstract
The induction of autophagy by nanoparticles causes nanotoxicity, but appropriate modulation of autophagy by nanoparticles may have therapeutic potential. Multiwalled carbon nanotubes (MWCNTs) interact with cell membranes and membrane-associated molecules before and after internalization. These interactions alter cellular signaling and impact major cell functions such as cell cycle, apoptosis, and autophagy. In this work, we demonstrated that MWCNT-cell interactions can be modulated by varying densely distributed surface ligands on MWCNTs. Using a fluorescent autophagy-reporting cell line, we evaluated the autophagy induction capability of 81 surface-modified MWCNTs. We identified strong and moderate autophagy-inducing MWCNTs as well as those that did not induce autophagy. Variation of the surface ligand structure of strong autophagy nanoinducers led to the induction of different autophagy-activating signaling pathways, presumably through their different interactions with cell surface receptors.
Original language | English (US) |
---|---|
Pages (from-to) | 2087-2099 |
Number of pages | 13 |
Journal | ACS Nano |
Volume | 8 |
Issue number | 3 |
DOIs | |
State | Published - Mar 25 2014 |
Externally published | Yes |
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Keywords
- autophagy
- carbon nanotube
- combinatorial library
- mTOR
- signaling pathway
- surface modification
ASJC Scopus subject areas
- Materials Science(all)
- Engineering(all)
- Physics and Astronomy(all)
Cite this
Tuning cell autophagy by diversifying carbon nanotube surface chemistry. / Wu, Ling; Zhang, Yi; Zhang, Chengke; Cui, Xuehui; Zhai, Shumei; Liu, Yin; Li, Changlong; Zhu, Hao; Qu, Guangbo; Jiang, Guibin; Yan, Bing.
In: ACS Nano, Vol. 8, No. 3, 25.03.2014, p. 2087-2099.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tuning cell autophagy by diversifying carbon nanotube surface chemistry
AU - Wu, Ling
AU - Zhang, Yi
AU - Zhang, Chengke
AU - Cui, Xuehui
AU - Zhai, Shumei
AU - Liu, Yin
AU - Li, Changlong
AU - Zhu, Hao
AU - Qu, Guangbo
AU - Jiang, Guibin
AU - Yan, Bing
PY - 2014/3/25
Y1 - 2014/3/25
N2 - The induction of autophagy by nanoparticles causes nanotoxicity, but appropriate modulation of autophagy by nanoparticles may have therapeutic potential. Multiwalled carbon nanotubes (MWCNTs) interact with cell membranes and membrane-associated molecules before and after internalization. These interactions alter cellular signaling and impact major cell functions such as cell cycle, apoptosis, and autophagy. In this work, we demonstrated that MWCNT-cell interactions can be modulated by varying densely distributed surface ligands on MWCNTs. Using a fluorescent autophagy-reporting cell line, we evaluated the autophagy induction capability of 81 surface-modified MWCNTs. We identified strong and moderate autophagy-inducing MWCNTs as well as those that did not induce autophagy. Variation of the surface ligand structure of strong autophagy nanoinducers led to the induction of different autophagy-activating signaling pathways, presumably through their different interactions with cell surface receptors.
AB - The induction of autophagy by nanoparticles causes nanotoxicity, but appropriate modulation of autophagy by nanoparticles may have therapeutic potential. Multiwalled carbon nanotubes (MWCNTs) interact with cell membranes and membrane-associated molecules before and after internalization. These interactions alter cellular signaling and impact major cell functions such as cell cycle, apoptosis, and autophagy. In this work, we demonstrated that MWCNT-cell interactions can be modulated by varying densely distributed surface ligands on MWCNTs. Using a fluorescent autophagy-reporting cell line, we evaluated the autophagy induction capability of 81 surface-modified MWCNTs. We identified strong and moderate autophagy-inducing MWCNTs as well as those that did not induce autophagy. Variation of the surface ligand structure of strong autophagy nanoinducers led to the induction of different autophagy-activating signaling pathways, presumably through their different interactions with cell surface receptors.
KW - autophagy
KW - carbon nanotube
KW - combinatorial library
KW - mTOR
KW - signaling pathway
KW - surface modification
UR - http://www.scopus.com/inward/record.url?scp=84896908426&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896908426&partnerID=8YFLogxK
U2 - 10.1021/nn500376w
DO - 10.1021/nn500376w
M3 - Article
C2 - 24552177
AN - SCOPUS:84896908426
VL - 8
SP - 2087
EP - 2099
JO - ACS Nano
JF - ACS Nano
SN - 1936-0851
IS - 3
ER -