Tunicamycin-mediated depletion of insulin receptors in 3T3-L1 adipocytes

O. M. Rosen, G. H. Chia, C. Fung, C. S. Rubin

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Tunicamycin, an antibiotic that inhibits protein glycosylation, elicited a rapid depletion of insulin binding activity at the surface of 3T3-L1 adipocytes. Disappearance of insulin receptors occurred more rapidly in the presence of tunicamycin than when protein synthesis was inhibited by cycloheximide and was accompanied by a diminution in sensitivity of the adipocytes to the acute effects of insulin and anti-insulin receptor antibody on hexose uptake and metabolism.

Original languageEnglish (US)
Pages (from-to)37-42
Number of pages6
JournalJournal of Cellular Physiology
Volume99
Issue number1
StatePublished - 1979

Fingerprint

Tunicamycin
Insulin Receptor
Adipocytes
Insulin
Glycosylation
Insulin Antibodies
Hexoses
Cycloheximide
Metabolism
Proteins
Anti-Bacterial Agents
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Rosen, O. M., Chia, G. H., Fung, C., & Rubin, C. S. (1979). Tunicamycin-mediated depletion of insulin receptors in 3T3-L1 adipocytes. Journal of Cellular Physiology, 99(1), 37-42.

Tunicamycin-mediated depletion of insulin receptors in 3T3-L1 adipocytes. / Rosen, O. M.; Chia, G. H.; Fung, C.; Rubin, C. S.

In: Journal of Cellular Physiology, Vol. 99, No. 1, 1979, p. 37-42.

Research output: Contribution to journalArticle

Rosen, OM, Chia, GH, Fung, C & Rubin, CS 1979, 'Tunicamycin-mediated depletion of insulin receptors in 3T3-L1 adipocytes', Journal of Cellular Physiology, vol. 99, no. 1, pp. 37-42.
Rosen, O. M. ; Chia, G. H. ; Fung, C. ; Rubin, C. S. / Tunicamycin-mediated depletion of insulin receptors in 3T3-L1 adipocytes. In: Journal of Cellular Physiology. 1979 ; Vol. 99, No. 1. pp. 37-42.
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