Tumorigenicity of green turtle fibropapilloma-derived fibroblast lines in immunodeficient mice

Lawrence H. Herbst, J. P. Sundberg, L. D. Shultz, B. A. Gray, P. A. Klein

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fibroblast lines derived from normal skin and spontaneous or experimentally induced fibropapillomas of green turtles (Chelonia mydas) were established and propagated in medium composed of a combination of Dulbecco's minimal essential with F12 medium plus 10% fetal bovine serum at 30°C. Fibropapilloma-derived fibroblasts were indistinguishable from normal skin fibroblasts in vitro. Tumor lines did not exhibit loss of contact inhibition, anchorage independence, or reduced serum requirements. Inoculation of primary and early-passage tumor cells into the medial margin of the pinna of C57BL/6J-nu/nu, C.B17-scid/scid, or NOD-scid/scid mice, however, resulted in fibroma formation, whereas inoculation of normal skin fibroblasts did not. Tumor-derived cells inoculated into the flanks of mice did not form tumors. The turtle origin of fibroblasts in tumors from mouse ears was confirmed by immunohistochemical and karyotype analysis. Fibroblast lines that were established from mouse ear fibromas had the normal karyotype (modal 2N = 55) of C. mydas. The cooler anatomic sites (ears) of immunodeficient mice are useful for confirming the tumorigenic (transformed) phenotype of green turtle fibropapillomatosis-derived fibroblasts. This mouse ear tumorigenicity test should facilitate studies of mechanisms of cellular transformation in green turtle fibropapillomatosis and other neoplastic diseases of poikilothermic vertebrates.

Original languageEnglish (US)
Pages (from-to)162-167
Number of pages6
JournalComparative Medicine
Volume48
Issue number2
StatePublished - 1998

Fingerprint

Chelonia mydas
Turtles
Fibroblasts
fibroblasts
Tumors
mice
Ear
ears
skin (animal)
fibroma
Skin
neoplasms
Fibroma
Neoplasms
Karyotype
karyotyping
Carcinogenicity Tests
Contact Inhibition
fetal bovine serum
blood serum

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Herbst, L. H., Sundberg, J. P., Shultz, L. D., Gray, B. A., & Klein, P. A. (1998). Tumorigenicity of green turtle fibropapilloma-derived fibroblast lines in immunodeficient mice. Comparative Medicine, 48(2), 162-167.

Tumorigenicity of green turtle fibropapilloma-derived fibroblast lines in immunodeficient mice. / Herbst, Lawrence H.; Sundberg, J. P.; Shultz, L. D.; Gray, B. A.; Klein, P. A.

In: Comparative Medicine, Vol. 48, No. 2, 1998, p. 162-167.

Research output: Contribution to journalArticle

Herbst, LH, Sundberg, JP, Shultz, LD, Gray, BA & Klein, PA 1998, 'Tumorigenicity of green turtle fibropapilloma-derived fibroblast lines in immunodeficient mice', Comparative Medicine, vol. 48, no. 2, pp. 162-167.
Herbst, Lawrence H. ; Sundberg, J. P. ; Shultz, L. D. ; Gray, B. A. ; Klein, P. A. / Tumorigenicity of green turtle fibropapilloma-derived fibroblast lines in immunodeficient mice. In: Comparative Medicine. 1998 ; Vol. 48, No. 2. pp. 162-167.
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