Tumor necrosis factor α induces adhesion molecule expression on human fetal astrocytes

Arthur A. Hurwitz, William D. Lyman, Michelle P. Guida, Tina M. Calderon, Joan W. Berman

Research output: Contribution to journalArticle

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Abstract

Leukocyte adhesion molecules on endothelial cells of the blood-brain barrier may participate in the entry of leukocytes into the central nervous system. Because astrocytes are also a component of the blood-brain barrier and have been associated with inflammation, we studied the ability of astrocytes to express leukocyte adhesion molecules using Northern blot and immunocytochemical techniques. Astrocytes treated with the proinflammatory cytokine tumor necrosis factor α (TNF) expressed messenger RNA for the adhesion molecules E-selectin, vascular cell adhesion molecule 1, and intercellular adhesion molecule 1, as well as their corresponding proteins. In addition, TNF-treated astrocytes expressed a monocyte adhesion protein identified by our laboratory, recognized by the monoclonal antibody IG9. These results indicate that under inflammatory conditions in the central nervous system, such as multiple sclerosis and acquired immune deficiency syndrome, astrocyte expression of adhesion molecules may facilitate the migration of leukocytes and contribute to the disease process.

Original languageEnglish (US)
Pages (from-to)1631-1636
Number of pages6
JournalJournal of Experimental Medicine
Volume176
Issue number6
StatePublished - Dec 1 1992

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Astrocytes
Tumor Necrosis Factor-alpha
Cell Adhesion Molecules
Blood-Brain Barrier
Leukocytes
Central Nervous System
E-Selectin
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Northern Blotting
Multiple Sclerosis
Monocytes
Acquired Immunodeficiency Syndrome
Proteins
Endothelial Cells
Monoclonal Antibodies
Cytokines
Inflammation
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Tumor necrosis factor α induces adhesion molecule expression on human fetal astrocytes. / Hurwitz, Arthur A.; Lyman, William D.; Guida, Michelle P.; Calderon, Tina M.; Berman, Joan W.

In: Journal of Experimental Medicine, Vol. 176, No. 6, 01.12.1992, p. 1631-1636.

Research output: Contribution to journalArticle

Hurwitz, Arthur A. ; Lyman, William D. ; Guida, Michelle P. ; Calderon, Tina M. ; Berman, Joan W. / Tumor necrosis factor α induces adhesion molecule expression on human fetal astrocytes. In: Journal of Experimental Medicine. 1992 ; Vol. 176, No. 6. pp. 1631-1636.
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