Tuberculosis genes expressed during persistence and reactivation in the resistant rabbit model

Anup K. Kesavan, Megan Brooks, Jo Ann Tufariello, John Chan, Yukari C. Manabe

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

As previously published, after aerosol infection with Mycobacterium tuberculosis H37Rv, New Zealand white rabbits established infection with active bacillary replication, but later contained disease to a paucibacillary state through an effective adaptive response consistent with latency. Despite the heterogeneity among outbred rabbits, the resistant response was uniform. Immunosuppression resulted in reactivation with increased lung bacillary burden. Using this rabbit model, we isolated bacillary RNA from infected rabbit lungs and assessed transcriptional profiles of bacillary genes using RT-PCR to examine genes differentially regulated during active replication, persistence, steroid-induced reactivation, and post-steroid immune reconstitution. Genes involved in hypoxia response (fdxA), resuscitation promoting factors (rpfB), and DNA repair pathways (Rv2191) may be important in bacillary persistence. Further investigation into these gene pathways is warranted.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalTuberculosis
Volume89
Issue number1
DOIs
StatePublished - Jan 1 2009

Keywords

  • Animal model
  • Gene expression
  • Latency
  • Mycobacterium tuberculosis
  • Rabbit
  • Reactivation

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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