Abstract
Initial studies have identified TSP50 as a human testes-specific gene that is demethylated in breast cancer. In this study, we will present new data related to the TSP50 gene. We have found that the TSP50 gene product shares a similar enzymatic structure with many serine proteases. However, the most critical catalytic site, serine, has been replaced by threonine. Western analysis revealed that in human testes, the TSP50 antibody detected two closely positioned protein bands whose estimated molecular masses were 37 kDa, whereas in a large portion of breast cancer tissues, but not normal control tissues, only one band was present. Immunohistochemistry assays found TSP50 proteins located in the spermatocytes of human testes, whereas in situ hybridization and immunohistochemistry confirmed that gene activation in breast tumors took place in malignant mammary epithelial cells. These results suggested that the normal function of the TSP50 gene was involved in spermatogenesis, whereas the up-regulation of TSP50 in many breast cancer patients not only indicated that it might be a novel biomarker for this disease but also encouraged us to further explore the possibility of whether it was an oncogene.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 290-294 |
| Number of pages | 5 |
| Journal | Cancer Research |
| Volume | 62 |
| Issue number | 1 |
| State | Published - Jan 1 2002 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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TSP50, a possible protease in human testes, is activated in breast cancer epithelial cells. / Shan, Jidong; Yuan, Liming; Xiao, Qianxun; Chiorazzi, Nicholas; Budman, Daniel; Teichberg, Saul; Xu, Hao Peng.
In: Cancer Research, Vol. 62, No. 1, 01.01.2002, p. 290-294.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - TSP50, a possible protease in human testes, is activated in breast cancer epithelial cells
AU - Shan, Jidong
AU - Yuan, Liming
AU - Xiao, Qianxun
AU - Chiorazzi, Nicholas
AU - Budman, Daniel
AU - Teichberg, Saul
AU - Xu, Hao Peng
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Initial studies have identified TSP50 as a human testes-specific gene that is demethylated in breast cancer. In this study, we will present new data related to the TSP50 gene. We have found that the TSP50 gene product shares a similar enzymatic structure with many serine proteases. However, the most critical catalytic site, serine, has been replaced by threonine. Western analysis revealed that in human testes, the TSP50 antibody detected two closely positioned protein bands whose estimated molecular masses were 37 kDa, whereas in a large portion of breast cancer tissues, but not normal control tissues, only one band was present. Immunohistochemistry assays found TSP50 proteins located in the spermatocytes of human testes, whereas in situ hybridization and immunohistochemistry confirmed that gene activation in breast tumors took place in malignant mammary epithelial cells. These results suggested that the normal function of the TSP50 gene was involved in spermatogenesis, whereas the up-regulation of TSP50 in many breast cancer patients not only indicated that it might be a novel biomarker for this disease but also encouraged us to further explore the possibility of whether it was an oncogene.
AB - Initial studies have identified TSP50 as a human testes-specific gene that is demethylated in breast cancer. In this study, we will present new data related to the TSP50 gene. We have found that the TSP50 gene product shares a similar enzymatic structure with many serine proteases. However, the most critical catalytic site, serine, has been replaced by threonine. Western analysis revealed that in human testes, the TSP50 antibody detected two closely positioned protein bands whose estimated molecular masses were 37 kDa, whereas in a large portion of breast cancer tissues, but not normal control tissues, only one band was present. Immunohistochemistry assays found TSP50 proteins located in the spermatocytes of human testes, whereas in situ hybridization and immunohistochemistry confirmed that gene activation in breast tumors took place in malignant mammary epithelial cells. These results suggested that the normal function of the TSP50 gene was involved in spermatogenesis, whereas the up-regulation of TSP50 in many breast cancer patients not only indicated that it might be a novel biomarker for this disease but also encouraged us to further explore the possibility of whether it was an oncogene.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036144601&partnerID=8YFLogxK
M3 - Article
C2 - 11782390
AN - SCOPUS:0036144601
VL - 62
SP - 290
EP - 294
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 1
ER -