TY - JOUR
T1 - Trypanosoma cruzi triggers an early type I IFN response in vivo at the site of intradermal infection
AU - Chessler, Anne Danielle C.
AU - Unnikrishnan, Meera
AU - Bei, Amy K.
AU - Daily, Johanna P.
AU - Burleigh, Barbara A.
PY - 2009/2/15
Y1 - 2009/2/15
N2 - Early interactions between the protozoan parasite Trypanosoma cruzi and mammalian hosts at primary sites of infection (skin and mucosal membranes) are predicted to be critical determinants of parasite survival and dissemination in the host. To investigate the early host response triggered by three different strains of T. cruzi at a local infection site, changes in host gene expression were monitored in a murine intradermal infection model using Affymetrix oligonucleotide arrays. Robust induction of IFN-stimulated genes was observed in excised skin 24 h postinfection where the level of IFN-stimulated gene induction was parasite straindependent, with the least virulent strain triggering a muted IFN response. Infection of mice immunodepleted of IFN-γ-producing cells or infection of IFN-γ-deficient mice had minimal impact on the IFN response generated in T. cruzi-infected mice. In contrast, infection of mice lacking the type I IFN receptor demonstrated that type I IFNs are largely responsible for the IFN response generated at the site of infection. These data highlight type I IFNs as important components of the innate immune response to T. cruzi at the site of inoculation and their role in shaping the early transcriptional response to this pathogen.
AB - Early interactions between the protozoan parasite Trypanosoma cruzi and mammalian hosts at primary sites of infection (skin and mucosal membranes) are predicted to be critical determinants of parasite survival and dissemination in the host. To investigate the early host response triggered by three different strains of T. cruzi at a local infection site, changes in host gene expression were monitored in a murine intradermal infection model using Affymetrix oligonucleotide arrays. Robust induction of IFN-stimulated genes was observed in excised skin 24 h postinfection where the level of IFN-stimulated gene induction was parasite straindependent, with the least virulent strain triggering a muted IFN response. Infection of mice immunodepleted of IFN-γ-producing cells or infection of IFN-γ-deficient mice had minimal impact on the IFN response generated in T. cruzi-infected mice. In contrast, infection of mice lacking the type I IFN receptor demonstrated that type I IFNs are largely responsible for the IFN response generated at the site of infection. These data highlight type I IFNs as important components of the innate immune response to T. cruzi at the site of inoculation and their role in shaping the early transcriptional response to this pathogen.
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U2 - 10.4049/jimmunol.0800621
DO - 10.4049/jimmunol.0800621
M3 - Article
C2 - 19201883
AN - SCOPUS:61449148441
SN - 0022-1767
VL - 182
SP - 2288
EP - 2296
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -