Trypanosoma cruzi Infection of cultured adipocytes results in an inflammatory phenotype

Fnu Nagajyothi, Mahalia S. Desruisseaux, Niranjan Thiruvur, Louis M. Weiss, Vicki L. Braunstein, Chris Albanese, Mauro M. Teixeira, Cecilia J. De Almeida, Michael P. Lisanti, Philipp E. Scherer, Herbert B. Tanowitz

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Abstract

Infection with Trypanosoma cruzi, the etiologic agent of Chagas disease is accompanied by an intense inflammatory reaction. Our laboratory group has identified adipose tissue as one of the major sites of inflammation during disease progression. Because adipose tissue is composed of many cell types, we were interested in investigating whether the adipocyte per se was a source of inflammatory mediators in this infection. Cultured adipocytes were infected with the Tulahuen strain of T. cruzi for 48-96 h. Immunoblot and quantitative PCR (qPCR) analyses demonstrated an increase in the expression of proinflammatory cytokines and chemokines, including interleukin (IL)-1Β, interferon-γ, tumor necrosis factor-α, CCL2, CCL5, and CXCL10 as well as an increase in the expression of Toll-like receptors-2 and 9 and activation of the notch pathway. Interestingly, caveolin-1 expression was reduced while cyclin D1 and extracellular signal-regulated kinase (ERK) expression was increased. The expression of PI3kinase and the activation of AKT (phosphorylated AKT) were increased suggesting that infection may induce components of the insulin/IGF-1 receptor cascade. There was an infection-associated decrease in adiponectin and peroxisome proliferator- activated receptor-γ (PPAR-γ). These data provide a mechanism for the increase in the inflammatory phenotype that occurs in T. cruzi-infected adipocytes. Overall, these data implicate the adipocyte as an important target of T. cruzi, and one which contributes significantly to the inflammatory response observed in Chagas disease.

Original languageEnglish (US)
Pages (from-to)1992-1997
Number of pages6
JournalObesity
Volume16
Issue number9
DOIs
StatePublished - Sep 2008

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Trypanosoma cruzi
Adipocytes
Phenotype
Chagas Disease
Infection
Adipose Tissue
Toll-Like Receptor 9
Caveolin 1
Toll-Like Receptor 2
IGF Type 1 Receptor
Peroxisome Proliferator-Activated Receptors
Adiponectin
Cyclin D1
Extracellular Signal-Regulated MAP Kinases
Interleukin-1
Chemokines
Interferons
Disease Progression
Tumor Necrosis Factor-alpha
Insulin

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Nagajyothi, F., Desruisseaux, M. S., Thiruvur, N., Weiss, L. M., Braunstein, V. L., Albanese, C., ... Tanowitz, H. B. (2008). Trypanosoma cruzi Infection of cultured adipocytes results in an inflammatory phenotype. Obesity, 16(9), 1992-1997. https://doi.org/10.1038/oby.2008.331

Trypanosoma cruzi Infection of cultured adipocytes results in an inflammatory phenotype. / Nagajyothi, Fnu; Desruisseaux, Mahalia S.; Thiruvur, Niranjan; Weiss, Louis M.; Braunstein, Vicki L.; Albanese, Chris; Teixeira, Mauro M.; De Almeida, Cecilia J.; Lisanti, Michael P.; Scherer, Philipp E.; Tanowitz, Herbert B.

In: Obesity, Vol. 16, No. 9, 09.2008, p. 1992-1997.

Research output: Contribution to journalArticle

Nagajyothi, F, Desruisseaux, MS, Thiruvur, N, Weiss, LM, Braunstein, VL, Albanese, C, Teixeira, MM, De Almeida, CJ, Lisanti, MP, Scherer, PE & Tanowitz, HB 2008, 'Trypanosoma cruzi Infection of cultured adipocytes results in an inflammatory phenotype', Obesity, vol. 16, no. 9, pp. 1992-1997. https://doi.org/10.1038/oby.2008.331
Nagajyothi F, Desruisseaux MS, Thiruvur N, Weiss LM, Braunstein VL, Albanese C et al. Trypanosoma cruzi Infection of cultured adipocytes results in an inflammatory phenotype. Obesity. 2008 Sep;16(9):1992-1997. https://doi.org/10.1038/oby.2008.331
Nagajyothi, Fnu ; Desruisseaux, Mahalia S. ; Thiruvur, Niranjan ; Weiss, Louis M. ; Braunstein, Vicki L. ; Albanese, Chris ; Teixeira, Mauro M. ; De Almeida, Cecilia J. ; Lisanti, Michael P. ; Scherer, Philipp E. ; Tanowitz, Herbert B. / Trypanosoma cruzi Infection of cultured adipocytes results in an inflammatory phenotype. In: Obesity. 2008 ; Vol. 16, No. 9. pp. 1992-1997.
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