Trypanosoma cruzi infection affects actin mRNA regulation in heart muscle cells

Mirian Claudia S Pereira, Robert H. Singer, Maria De Nazareth S L De Meirelles

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have previously described alterations in the cytoskeletal organization of heart muscle cells (HMC) infected with Trypanosoma cruzi in vitro. Our aim was to investigate whether these changes also affect the regulation of the actin mRNAs during HMC differentiation. Northern blot analysis revealed that α-cardiac actin mRNA levels increased during cell differentiation while β-actin mRNA levels declined. Nonmuscle cells displayed β-actin mRNA signal localized at the cell periphery, while α-cardiac actin mRNA had a perinuclear distribution in myocytes. Trypanosoma cruzi-infected cells showed 50% reduction in α-cardiac actin mRNA expression after 72 h of infection. In contrast, β-actin mRNA levels increased approximately 79% after 48 h of infection. In addition, in situ β-actin mRNA was delocalized from the periphery into the perinuclear region. These observations support the hypothesis that Trypanosoma cruzi affects actin mRNA regulation and localization through its effect on the cytoskeleton of heart muscle cells.

Original languageEnglish (US)
Pages (from-to)271-279
Number of pages9
JournalJournal of Eukaryotic Microbiology
Volume47
Issue number3
StatePublished - 2000

Fingerprint

Trypanosoma cruzi
Cardiac Myocytes
myocytes
actin
Actins
heart
Messenger RNA
Infection
infection
cell differentiation
Cell Differentiation
cells
cytoskeleton
Cytoskeleton
Northern blotting
Northern Blotting
Muscle Cells

Keywords

  • Actin mRNA
  • Cardiomyocytes
  • In situ hybridization
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Cite this

Trypanosoma cruzi infection affects actin mRNA regulation in heart muscle cells. / Pereira, Mirian Claudia S; Singer, Robert H.; De Meirelles, Maria De Nazareth S L.

In: Journal of Eukaryotic Microbiology, Vol. 47, No. 3, 2000, p. 271-279.

Research output: Contribution to journalArticle

Pereira, Mirian Claudia S ; Singer, Robert H. ; De Meirelles, Maria De Nazareth S L. / Trypanosoma cruzi infection affects actin mRNA regulation in heart muscle cells. In: Journal of Eukaryotic Microbiology. 2000 ; Vol. 47, No. 3. pp. 271-279.
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