Trypanosoma cruzi: Colony formation and clonal growth in agar

Murray Wittner, Linda Squillante, Jeffrey P. Nadler, Herbert B. Tanowitz

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Trypanosoma cruzi exhibited colonial growth when incorporated into 0.5-0.6% agar. Colonies were established from a single organism and clones readily derived. The plating efficiencies were variable depending on the original inoculum but were consistently over 50% when 104 to 105 parasites were added. The use of this technique for evaluation of an antitrypanosomal agent, nifurtimox, was demonstrated, making possible large-scale testing of potential antitrypanosomal agents and assessment of microbicidal and microbio-static drug levels.

Original languageEnglish (US)
Pages (from-to)255-261
Number of pages7
JournalExperimental Parasitology
Volume53
Issue number2
DOIs
Publication statusPublished - Apr 1982

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Keywords

  • Agar medium
  • Chagas' disease
  • Cloning
  • Colony formation
  • Drug screening
  • Hemoflagellate
  • Nifurtimox
  • Protozoa
  • Trypanosoma cruzi
  • parasitic

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

Cite this

Wittner, M., Squillante, L., Nadler, J. P., & Tanowitz, H. B. (1982). Trypanosoma cruzi: Colony formation and clonal growth in agar. Experimental Parasitology, 53(2), 255-261. https://doi.org/10.1016/0014-4894(82)90067-4