Trypanosoma cruzi: Colony formation and clonal growth in agar

Murray Wittner, Linda Squillante, Jeffrey P. Nadler, Herbert B. Tanowitz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Trypanosoma cruzi exhibited colonial growth when incorporated into 0.5-0.6% agar. Colonies were established from a single organism and clones readily derived. The plating efficiencies were variable depending on the original inoculum but were consistently over 50% when 104 to 105 parasites were added. The use of this technique for evaluation of an antitrypanosomal agent, nifurtimox, was demonstrated, making possible large-scale testing of potential antitrypanosomal agents and assessment of microbicidal and microbio-static drug levels.

Original languageEnglish (US)
Pages (from-to)255-261
Number of pages7
JournalExperimental Parasitology
Volume53
Issue number2
DOIs
StatePublished - 1982

Fingerprint

Nifurtimox
Trypanosoma cruzi
Agar
Parasites
Clone Cells
Growth
Pharmaceutical Preparations

Keywords

  • Agar medium
  • Chagas' disease
  • Cloning
  • Colony formation
  • Drug screening
  • Hemoflagellate
  • Nifurtimox
  • parasitic
  • Protozoa
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

Wittner, M., Squillante, L., Nadler, J. P., & Tanowitz, H. B. (1982). Trypanosoma cruzi: Colony formation and clonal growth in agar. Experimental Parasitology, 53(2), 255-261. https://doi.org/10.1016/0014-4894(82)90067-4

Trypanosoma cruzi : Colony formation and clonal growth in agar. / Wittner, Murray; Squillante, Linda; Nadler, Jeffrey P.; Tanowitz, Herbert B.

In: Experimental Parasitology, Vol. 53, No. 2, 1982, p. 255-261.

Research output: Contribution to journalArticle

Wittner, M, Squillante, L, Nadler, JP & Tanowitz, HB 1982, 'Trypanosoma cruzi: Colony formation and clonal growth in agar', Experimental Parasitology, vol. 53, no. 2, pp. 255-261. https://doi.org/10.1016/0014-4894(82)90067-4
Wittner, Murray ; Squillante, Linda ; Nadler, Jeffrey P. ; Tanowitz, Herbert B. / Trypanosoma cruzi : Colony formation and clonal growth in agar. In: Experimental Parasitology. 1982 ; Vol. 53, No. 2. pp. 255-261.
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