Trypanosoma cruzi-cardiomyocytes: New Contributions Regarding a Better Understanding of this Interaction

Maria De Nazareth Leal De Meirelles; Mirian Claudia S. Pereira; Robert H. Singer; Maria De Nazaré C. Soeiro; Luciana R. Garzoni; Dayse T. Silva; Helene S. Barbosa; Tania C. Araujo-Jorge; Masako O. Masuda; Marcia A.M. Capella; Anibal Gil Lopes; Alane B. Vermelho

doi:10.1590/s0074-02761999000700017

Trypanosoma cruzi-cardiomyocytes: New Contributions Regarding a Better Understanding of this Interaction

Maria De Nazareth Leal De Meirelles, Mirian Claudia S. Pereira, Robert H. Singer, Maria De Nazaré C. Soeiro, Luciana R. Garzoni, Dayse T. Silva, Helene S. Barbosa, Tania C. Araujo-Jorge, Masako O. Masuda, Marcia A.M. Capella, Anibal Gil Lopes, Alane B. Vermelho

Cell Biology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The present paper summarizes new approaches regarding the progress done to the understanding of the interaction of Trypanosoma cruzi-cardiomyocytes. Mannose receptors localized at the surface of heart muscle cell are involved in binding and uptake of the parasite. One of the most striking events in the parasite-heart muscle cells interaction is the disruption of the actin cytoskeleton. We have investigated the regulation of the actin mRNA during the cytopathology induced in myocardial cells by the parasite. T. cruzi invasion increases calcium resting levels in cardiomyocytes. We have previously shown that Ca²⁺ ATPase of the sarcoplasmic reticulum (SERCA) is involved in the invasion of T. cruzi in cardiomyocytes. Treating the cells with thapsigargin, a drug that binds to all SERCA ATPases and causes depletion of intracellular calcium stores, we found a 75% inhibition in the T. cruzi-cardiomyocytes invasion.

Original language	English (US)
Pages (from-to)	149-152
Number of pages	4
Journal	Memorias do Instituto Oswaldo Cruz
Volume	94
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1590/s0074-02761999000700017
State	Published - 1999

Keywords

Calcium
Cardiomyocytes
Mannose receptor
SERCA ATPases
Trypanosoma cruzi
mRNA regulation

ASJC Scopus subject areas

Microbiology (medical)

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10.1590/s0074-02761999000700017

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De Meirelles, M. D. N. L., Pereira, M. C. S., Singer, R. H., Soeiro, M. D. N. C., Garzoni, L. R., Silva, D. T., Barbosa, H. S., Araujo-Jorge, T. C., Masuda, M. O., Capella, M. A. M., Lopes, A. G., & Vermelho, A. B. (1999). Trypanosoma cruzi-cardiomyocytes: New Contributions Regarding a Better Understanding of this Interaction. Memorias do Instituto Oswaldo Cruz, 94(SUPPL. 1), 149-152. https://doi.org/10.1590/s0074-02761999000700017

De Meirelles, MDNL, Pereira, MCS, Singer, RH, Soeiro, MDNC, Garzoni, LR, Silva, DT, Barbosa, HS, Araujo-Jorge, TC, Masuda, MO, Capella, MAM, Lopes, AG & Vermelho, AB 1999, 'Trypanosoma cruzi-cardiomyocytes: New Contributions Regarding a Better Understanding of this Interaction', Memorias do Instituto Oswaldo Cruz, vol. 94, no. SUPPL. 1, pp. 149-152. https://doi.org/10.1590/s0074-02761999000700017

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abstract = "The present paper summarizes new approaches regarding the progress done to the understanding of the interaction of Trypanosoma cruzi-cardiomyocytes. Mannose receptors localized at the surface of heart muscle cell are involved in binding and uptake of the parasite. One of the most striking events in the parasite-heart muscle cells interaction is the disruption of the actin cytoskeleton. We have investigated the regulation of the actin mRNA during the cytopathology induced in myocardial cells by the parasite. T. cruzi invasion increases calcium resting levels in cardiomyocytes. We have previously shown that Ca2+ ATPase of the sarcoplasmic reticulum (SERCA) is involved in the invasion of T. cruzi in cardiomyocytes. Treating the cells with thapsigargin, a drug that binds to all SERCA ATPases and causes depletion of intracellular calcium stores, we found a 75% inhibition in the T. cruzi-cardiomyocytes invasion.",

keywords = "Calcium, Cardiomyocytes, Mannose receptor, SERCA ATPases, Trypanosoma cruzi, mRNA regulation",

author = "{De Meirelles}, {Maria De Nazareth Leal} and Pereira, {Mirian Claudia S.} and Singer, {Robert H.} and Soeiro, {Maria De Nazar{\'e} C.} and Garzoni, {Luciana R.} and Silva, {Dayse T.} and Barbosa, {Helene S.} and Araujo-Jorge, {Tania C.} and Masuda, {Masako O.} and Capella, {Marcia A.M.} and Lopes, {Anibal Gil} and Vermelho, {Alane B.}",

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AU - De Meirelles, Maria De Nazareth Leal

AU - Pereira, Mirian Claudia S.

AU - Singer, Robert H.

AU - Soeiro, Maria De Nazaré C.

AU - Garzoni, Luciana R.

AU - Silva, Dayse T.

AU - Barbosa, Helene S.

AU - Araujo-Jorge, Tania C.

AU - Masuda, Masako O.

AU - Capella, Marcia A.M.

AU - Lopes, Anibal Gil

AU - Vermelho, Alane B.

PY - 1999

Y1 - 1999

N2 - The present paper summarizes new approaches regarding the progress done to the understanding of the interaction of Trypanosoma cruzi-cardiomyocytes. Mannose receptors localized at the surface of heart muscle cell are involved in binding and uptake of the parasite. One of the most striking events in the parasite-heart muscle cells interaction is the disruption of the actin cytoskeleton. We have investigated the regulation of the actin mRNA during the cytopathology induced in myocardial cells by the parasite. T. cruzi invasion increases calcium resting levels in cardiomyocytes. We have previously shown that Ca2+ ATPase of the sarcoplasmic reticulum (SERCA) is involved in the invasion of T. cruzi in cardiomyocytes. Treating the cells with thapsigargin, a drug that binds to all SERCA ATPases and causes depletion of intracellular calcium stores, we found a 75% inhibition in the T. cruzi-cardiomyocytes invasion.

AB - The present paper summarizes new approaches regarding the progress done to the understanding of the interaction of Trypanosoma cruzi-cardiomyocytes. Mannose receptors localized at the surface of heart muscle cell are involved in binding and uptake of the parasite. One of the most striking events in the parasite-heart muscle cells interaction is the disruption of the actin cytoskeleton. We have investigated the regulation of the actin mRNA during the cytopathology induced in myocardial cells by the parasite. T. cruzi invasion increases calcium resting levels in cardiomyocytes. We have previously shown that Ca2+ ATPase of the sarcoplasmic reticulum (SERCA) is involved in the invasion of T. cruzi in cardiomyocytes. Treating the cells with thapsigargin, a drug that binds to all SERCA ATPases and causes depletion of intracellular calcium stores, we found a 75% inhibition in the T. cruzi-cardiomyocytes invasion.

KW - Calcium

KW - Cardiomyocytes

KW - Mannose receptor

KW - SERCA ATPases

KW - Trypanosoma cruzi

KW - mRNA regulation

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Trypanosoma cruzi-cardiomyocytes: New Contributions Regarding a Better Understanding of this Interaction

Abstract

Keywords

ASJC Scopus subject areas

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