TY - JOUR
T1 - TRPV4-mediated detection of hyposmotic stress by skin keratinocytes activates developmental immunity
AU - Galindo-Villegas, Jorge
AU - Montalban-Arques, Ana
AU - Liarte, Sergio
AU - De Oliveira, Sofia
AU - Pardo-Pastor, Carlos
AU - Rubio-Moscardo, Fanny
AU - Meseguer, Jose
AU - Valverde, Miguel A.
AU - Mulero, Victoriano
N1 - Publisher Copyright:
© 2016 by The American Association of Immunologists, Inc.
PY - 2016/1/15
Y1 - 2016/1/15
N2 - As an organism is exposed to pathogens during very early development, specific defense mechanisms must take effect. In this study, we used a germ-free zebrafish embryo model to show that osmotic stress regulates the activation of immunity and host protection in newly hatched embryos.Mechanistically, skin keratinocytes were responsible for both sensing the hyposmolarity of the aquatic environment and mediating immune effector mechanisms. This occurred through a transient potential receptor vanilloid 4/Ca2+/TGF-β-activated kinase 1/NF-κB signaling pathway. Surprisingly, the genes encoding antimicrobial effectors, which do not have the potential to cause tissue damage, are constitutively expressed during development, independently of both commensal microbes and osmotic stress. Our results reveal that osmotic stress is associated with the induction of developmental immunity in the absence of tissue damage and point out to the embryo skin as the first organ with full capacities to mount an innate immune response.
AB - As an organism is exposed to pathogens during very early development, specific defense mechanisms must take effect. In this study, we used a germ-free zebrafish embryo model to show that osmotic stress regulates the activation of immunity and host protection in newly hatched embryos.Mechanistically, skin keratinocytes were responsible for both sensing the hyposmolarity of the aquatic environment and mediating immune effector mechanisms. This occurred through a transient potential receptor vanilloid 4/Ca2+/TGF-β-activated kinase 1/NF-κB signaling pathway. Surprisingly, the genes encoding antimicrobial effectors, which do not have the potential to cause tissue damage, are constitutively expressed during development, independently of both commensal microbes and osmotic stress. Our results reveal that osmotic stress is associated with the induction of developmental immunity in the absence of tissue damage and point out to the embryo skin as the first organ with full capacities to mount an innate immune response.
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U2 - 10.4049/jimmunol.1501729
DO - 10.4049/jimmunol.1501729
M3 - Article
C2 - 26673139
AN - SCOPUS:84954095010
SN - 0022-1767
VL - 196
SP - 738
EP - 749
JO - Journal of Immunology
JF - Journal of Immunology
IS - 2
ER -