Tropoelastin inhibits intimal hyperplasia of mouse bioresorbable arterial vascular grafts

Tadahisa Sugiura, Riddhima Agarwal, Shuhei Tara, Tai Yi, Yong Ung Lee, Christopher K. Breuer, Anthony S. Weiss, Toshiharu Shinoka

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Neointimal hyperplasia, which results from the activation, proliferation and migration of vascular smooth muscle cells (SMCs), is a detrimental condition for vascular stents or vascular grafts that leads to stenosis. Preventing neointimal hyperplasia of vascular grafts is critically important for the success of arterial vascular grafts. We hypothesized that tropoelastin seeding onto the luminal surface of the graft would prevent neointimal hyperplasia through suppressing neointimal smooth muscle cell proliferation. In this study, we investigated the efficacy of tropoelastin seeding in preventing neointimal hyperplasia of bioresorbable arterial vascular grafts. Poly (glycolic acid) (PGA) fiber mesh coated with poly (L-lactic-co-ε-caprolactone) (PLCL) scaffolds reinforced by poly (L-lactic acid) (PLA) nano-fibers were prepared as bioresorbable arterial grafts. Tropoelastin was then seeded onto the luminal surface of the grafts. Tropoelastin significantly reduced the thickness of the intimal layer. This effect was mainly due to a substantial reduction the number of cells that stained positive for SMC (α-SMA) and PCNA in the vessel walls. Mature elastin and collagen type I and III were unchanged with tropoelastin treatment. This study demonstrates that tropoelastin seeding is beneficial in preventing SMC proliferation and neointimal hyperplasia in bioresorbable arterial vascular grafts. Statement of Significance Small resorbable vascular grafts can block due to the over-proliferation of smooth muscle cells in neointimal hyperplasia. We show here that the proliferation of these cells is restricted in this type of graft. This is achieved with a simple dip, non-covalent coating of tropoelastin. It is in principle amendable to other grafts and is therefore an attractive process. This study is particularly significant because: (1) it shows that smooth muscle cell proliferation can be reduced while still accommodating the growth of endothelial cells, (2) small vascular grafts with an internal diameter of less than 1 mm are amenable to this process, and (3) this process works for resorbable grafts.

Original languageEnglish (US)
Pages (from-to)74-80
Number of pages7
JournalActa Biomaterialia
Volume52
DOIs
StatePublished - Apr 1 2017
Externally publishedYes

Keywords

  • Bioresorbable vascular grafts
  • Intimal hyperplasia
  • Small diameter arterial grafts
  • Smooth muscle cells
  • Tropoelastin

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Fingerprint Dive into the research topics of 'Tropoelastin inhibits intimal hyperplasia of mouse bioresorbable arterial vascular grafts'. Together they form a unique fingerprint.

  • Cite this

    Sugiura, T., Agarwal, R., Tara, S., Yi, T., Lee, Y. U., Breuer, C. K., Weiss, A. S., & Shinoka, T. (2017). Tropoelastin inhibits intimal hyperplasia of mouse bioresorbable arterial vascular grafts. Acta Biomaterialia, 52, 74-80. https://doi.org/10.1016/j.actbio.2016.12.044