Trichophyton rubrum is inhibited by free and nanoparticle encapsulated curcumin by induction of nitrosative stress after photodynamic activation

Ludmila Matos Baltazar, Aimee E. Krausz, Ana Camila Oliveira Souza, Brandon L. Adler, Angelo Landriscina, Tagai Musaev, Joshua D. Nosanchuk, Adam J. Friedman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Antimicrobial photodynamic inhibition (aPI) utilizes radical stress generated from the excitation of a photosensitizer (PS) with light to destroy pathogens. Its use against Trichophyton rubrum, a dermatophytic fungus with increasing incidence and resistance, has not been well characterized. Our aim was to evaluate the mechanism of action of aPI against T. rubrum using curcumin as the PS in both free and nanoparticle (curc-np) form. Nanocarriers stabilize curcumin and allow for enhanced solubility and PS delivery. Curcumin aPI, at optimal conditions of 10 μg/mL of PS with 10 J/cm2 of blue light (417 ± 5 nm), completely inhibited fungal growth (p<0.0001) via induction of reactive oxygen (ROS) and nitrogen species (RNS), which was associated with fungal death by apoptosis. Interestingly, only scavengers of RNS impeded aPI efficacy, suggesting that curcumin acts potently via a nitrosative pathway. The curc-np induced greater NO expression and enhanced apoptosis of fungal cells, highlighting curc-np aPI as a potential treatment for T. rubrum skin infections.

Original languageEnglish (US)
Article numbere0120179
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 24 2015

Fingerprint

Trichophyton rubrum
Trichophyton
Curcumin
Photosensitizing Agents
curcumin
nanoparticles
Nanoparticles
anti-infective agents
Chemical activation
Nitrogen
Apoptosis
Light
Reactive Nitrogen Species
apoptosis
Pathogens
Fungi
Solubility
nitrogen
Reactive Oxygen Species
blue light

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Baltazar, L. M., Krausz, A. E., Souza, A. C. O., Adler, B. L., Landriscina, A., Musaev, T., ... Friedman, A. J. (2015). Trichophyton rubrum is inhibited by free and nanoparticle encapsulated curcumin by induction of nitrosative stress after photodynamic activation. PLoS One, 10(3), [e0120179]. https://doi.org/10.1371/journal.pone.0120179

Trichophyton rubrum is inhibited by free and nanoparticle encapsulated curcumin by induction of nitrosative stress after photodynamic activation. / Baltazar, Ludmila Matos; Krausz, Aimee E.; Souza, Ana Camila Oliveira; Adler, Brandon L.; Landriscina, Angelo; Musaev, Tagai; Nosanchuk, Joshua D.; Friedman, Adam J.

In: PLoS One, Vol. 10, No. 3, e0120179, 24.03.2015.

Research output: Contribution to journalArticle

Baltazar, Ludmila Matos ; Krausz, Aimee E. ; Souza, Ana Camila Oliveira ; Adler, Brandon L. ; Landriscina, Angelo ; Musaev, Tagai ; Nosanchuk, Joshua D. ; Friedman, Adam J. / Trichophyton rubrum is inhibited by free and nanoparticle encapsulated curcumin by induction of nitrosative stress after photodynamic activation. In: PLoS One. 2015 ; Vol. 10, No. 3.
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