Trehalose-Based Glassy Matrices as an Effective Tool to Trap Short-Lived Intermediates in the Nitric Oxide Dioxygenation (NOD) Reaction of Hemoglobin

Mahantesh S. Navati, Will Chung, Joel M. Friedman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The very rapid nitric oxide dioxygenation (NOD) reaction of nitric oxide (NO) with the oxygen bound to the ferrous derivatives of hemeproteins such as hemoglobin and myoglobin to yield nitrate and the ferric derivate (met) of the hemeprotein is of considerable physiological and biomedical importance. The mechanism for this reaction has been elusive due to the rapidity of the reaction. This article describes a method based both on using trehalose-derived glassy matrices to control the reaction of NO with oxyhemoglobin through both a temperature and glass-dependent modulation of the protein dynamics and a novel method of generating NO within the glassy matrix. The results support models in which there is a very rapid formation of an intermediate that immediately decays into an initial nonequilibrium population of high and low ferric nitrate that on a slower time scale relaxed to an easily dissociated equilibrium form of the ferric nitrate derivative of hemoglobin.

Original languageEnglish (US)
Pages (from-to)4529-4539
Number of pages11
JournalJournal of Physical Chemistry B
Volume120
Issue number20
DOIs
StatePublished - May 26 2016

Fingerprint

Trehalose
Hemoglobin
Nitric oxide
hemoglobin
nitric oxide
Nitric Oxide
Hemoglobins
traps
Hemeproteins
nitrates
Nitrates
matrices
oxyhemoglobin
Derivatives
Oxyhemoglobins
myoglobin
Myoglobin
Modulation
Oxygen
proteins

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Trehalose-Based Glassy Matrices as an Effective Tool to Trap Short-Lived Intermediates in the Nitric Oxide Dioxygenation (NOD) Reaction of Hemoglobin. / Navati, Mahantesh S.; Chung, Will; Friedman, Joel M.

In: Journal of Physical Chemistry B, Vol. 120, No. 20, 26.05.2016, p. 4529-4539.

Research output: Contribution to journalArticle

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