Treatment of experimental pancreatic cancer with 213-Bismuth-labeled chimeric antibody to single-strand DNA

Ruth A. Bryan, Zewei Jiang, Thomas Jandl, Julius Strauss, Wade Koba, Chukwuemeka Onyedika, Alfred Morgenstern, Frank Bruchertseifer, Alan L. Epstein, Ekaterina Dadachova

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Background: Novel approaches to treatment of pancreatic cancer (PCa) are urgently needed. A chimeric monoclonal antibody (mAb) chTNT3 binds to single-strand DNA (ssDNA) and RNA released from the non-viable cells in fast growing tumors. Here the authors investigated whether radioimmunotherapy (RIT) using chTNT3 mAb radiolabeled with 213-Bismuth (213Bi) could be effective in treatment of experimental PCa. Methods: Two human PCa cell lines, Panc1 and MiaPaCa-2, were used for in vitro experiments. The xenografts in mice were established using MiaPaCa-2 cells. Therapy compared 213Bi-chTNT3 (700 μCi) to gemcitabine or cisplatin, untreated controls and 'cold' chTNT3. Results: RIT abrogated the tumors growth while tumors in control groups grew aggressively. Chemotherapy was less effective than RIT and toxic to mice while RIT did not have any side effects. Conclusions: RIT with 213Bi-chTNT3 was safe and effective in the treatment of experimental PCa in comparison with chemotherapy. This makes α-RIT targeting ssDNA a promising modality for further development.

Original languageEnglish (US)
Pages (from-to)1243-1249
Number of pages7
JournalExpert Review of Anticancer Therapy
Volume14
Issue number10
DOIs
StatePublished - Oct 1 2014

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Keywords

  • 213-Bismuth
  • cisplatin
  • gemcitabine
  • intracellular antigens
  • pancreatic cancer
  • radioimmunotherapy
  • single-strand DNA

ASJC Scopus subject areas

  • Oncology
  • Pharmacology (medical)

Cite this

Bryan, R. A., Jiang, Z., Jandl, T., Strauss, J., Koba, W., Onyedika, C., Morgenstern, A., Bruchertseifer, F., Epstein, A. L., & Dadachova, E. (2014). Treatment of experimental pancreatic cancer with 213-Bismuth-labeled chimeric antibody to single-strand DNA. Expert Review of Anticancer Therapy, 14(10), 1243-1249. https://doi.org/10.1586/14737140.2014.952285