Treadmill-based task for assessing spatial memory in head-fixed mice

Jake T. Jordan, Kelsey D. McDermott, M. Agustina Frechou, Matthew Shtrahman, J. Tiago Gonçalves

Research output: Contribution to journalArticlepeer-review


Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, training mice on a rewarded behavioral task, and analyzing behavioral data. We discuss common problems and solutions we have developed to optimize training. Finally, we demonstrate how to test spatial memory performance using this task.

Original languageEnglish (US)
Article number100770
JournalSTAR Protocols
Issue number3
StatePublished - Sep 17 2021


  • Behavior
  • Microscopy
  • Model Organisms
  • Neuroscience

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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