Treadmill-based task for assessing spatial memory in head-fixed mice

Jake T. Jordan; Kelsey D. McDermott; M. Agustina Frechou; Matthew Shtrahman; J. Tiago Gonçalves

doi:10.1016/j.xpro.2021.100770

Treadmill-based task for assessing spatial memory in head-fixed mice

Jake T. Jordan, Kelsey D. McDermott, M. Agustina Frechou, Matthew Shtrahman, J. Tiago Gonçalves

Neuroscience

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, training mice on a rewarded behavioral task, and analyzing behavioral data. We discuss common problems and solutions we have developed to optimize training. Finally, we demonstrate how to test spatial memory performance using this task.

Original language	English (US)
Article number	100770
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100770
State	Published - Sep 17 2021

Keywords

Behavior
Microscopy
Model Organisms
Neuroscience

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2021.100770

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abstract = "Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, training mice on a rewarded behavioral task, and analyzing behavioral data. We discuss common problems and solutions we have developed to optimize training. Finally, we demonstrate how to test spatial memory performance using this task.",

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AU - Gonçalves, J. Tiago

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Treadmill-based task for assessing spatial memory in head-fixed mice

Abstract

Keywords

ASJC Scopus subject areas

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