Transplantation of Wharton's jelly alleviates inflammatory response and depression after traumatic brain injury in mice

Tian Cheng, Wenwen Liu, Shanshan Ma, Tongwen Sun, Fangxia Guan

Research output: Contribution to journalArticle

Abstract

Objective To investigate the influence of Wharton's jelly (WJ) transplant on brain inflammation and mood status in traumatic brain injury (TBI) mouse model. Methods The WJ was isolated from human umbilical cord and cultured, and the cell phenotype of P3 human umbilical cord mesenchymal stem cells was identified by flow cytometry.The animal model was established by modified weight drop method. Experimental mice were randomly(random number) divided into Veh(normal saline) and WJ transplantation groups. After 3 days, water content of damaged brain was detected. Elisa kit was used to detect the expression of IL-1 p and TNF-a. The expression of GFAP, Ibal and CD68 were detected by immunofluorescence. Het(hydroethidine) staining was used to detect reactive oxygen species (ROS) production. Neurologic deficit score was used to evaluate the motor function, sucrose preference test, tail suspension test and forced swim test were used to detect the depression of mice.The data were expressed in (x±s) and analysēd by SPSS 21.0 software. Two-way ANOVA with repeated measures design was used to compare difference bewteen two groups at multiple interval. Results Compared with Veh group, the mice in the WJ group had a better performance in NDS scored test(dl: 14.8±1.169 vs. 15.2±1.472); 3d: (U.0±1.414 vs. 13.5±1.225); 7d: (9.5±1.517 vs. 12.0±1.549); 14d: (7.7±0.816 vs. 10.5±1.643); 21d: (6.5±0.547 vs. 9.0±1.265); 28d: (5.3±0.816 vs. 7.8±1.169), P<0.05]. After TBI, WJ tissue transplantation increased sucrose preference index from (54.49± 1.505)% to (64.56±2.279)% (P=0.004), decreased immobility time using tail suspension test from (144.7±5.493)s to (115.7±4.660)s (P<0.01), and decreased immobility time using forced swim test from (260.3±4.558)s to (215.8±5.003)s (P=0.002). After WJ transplantation, brain water content was reduced from (84.48±1.802)% to (75.58±1.559)% (P=0.004), the expression of IL-1β and TNF-α near injury area also decreased(P=0.000 6 and 0.000 3), as well as the expression of ROS(P=0.020). The fluorescence intensity of activated astrocytes decreased from (2 906±431.591)to (165 8±312.912) (P=0.041), and the number of microglias and activated microglias were both reduced(P=0.049 and P<0.01) after TBI. Conclusions Wharton's jelly alleviated the inflammation and depression in traumatic brain injured mice.

Original languageEnglish (US)
Pages (from-to)779-784
Number of pages6
JournalChinese Journal of Emergency Medicine
Volume27
Issue number7
DOIs
StatePublished - Jul 1 2018
Externally publishedYes

Fingerprint

Wharton Jelly
Transplantation
Depression
Hindlimb Suspension
Umbilical Cord
Interleukin-1
Sucrose
Reactive Oxygen Species
Brain
Tissue Transplantation
Water
Encephalitis
Neurologic Manifestations
Traumatic Brain Injury
Mesenchymal Stromal Cells
Astrocytes
Fluorescent Antibody Technique
Cultured Cells
Analysis of Variance
Flow Cytometry

Keywords

  • Depression
  • Inflammation
  • Traumatic brain injury
  • Wharton's jelly

ASJC Scopus subject areas

  • Emergency Medicine
  • Emergency

Cite this

Transplantation of Wharton's jelly alleviates inflammatory response and depression after traumatic brain injury in mice. / Cheng, Tian; Liu, Wenwen; Ma, Shanshan; Sun, Tongwen; Guan, Fangxia.

In: Chinese Journal of Emergency Medicine, Vol. 27, No. 7, 01.07.2018, p. 779-784.

Research output: Contribution to journalArticle

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abstract = "Objective To investigate the influence of Wharton's jelly (WJ) transplant on brain inflammation and mood status in traumatic brain injury (TBI) mouse model. Methods The WJ was isolated from human umbilical cord and cultured, and the cell phenotype of P3 human umbilical cord mesenchymal stem cells was identified by flow cytometry.The animal model was established by modified weight drop method. Experimental mice were randomly(random number) divided into Veh(normal saline) and WJ transplantation groups. After 3 days, water content of damaged brain was detected. Elisa kit was used to detect the expression of IL-1 p and TNF-a. The expression of GFAP, Ibal and CD68 were detected by immunofluorescence. Het(hydroethidine) staining was used to detect reactive oxygen species (ROS) production. Neurologic deficit score was used to evaluate the motor function, sucrose preference test, tail suspension test and forced swim test were used to detect the depression of mice.The data were expressed in (x±s) and analysēd by SPSS 21.0 software. Two-way ANOVA with repeated measures design was used to compare difference bewteen two groups at multiple interval. Results Compared with Veh group, the mice in the WJ group had a better performance in NDS scored test(dl: 14.8±1.169 vs. 15.2±1.472); 3d: (U.0±1.414 vs. 13.5±1.225); 7d: (9.5±1.517 vs. 12.0±1.549); 14d: (7.7±0.816 vs. 10.5±1.643); 21d: (6.5±0.547 vs. 9.0±1.265); 28d: (5.3±0.816 vs. 7.8±1.169), P<0.05]. After TBI, WJ tissue transplantation increased sucrose preference index from (54.49± 1.505){\%} to (64.56±2.279){\%} (P=0.004), decreased immobility time using tail suspension test from (144.7±5.493)s to (115.7±4.660)s (P<0.01), and decreased immobility time using forced swim test from (260.3±4.558)s to (215.8±5.003)s (P=0.002). After WJ transplantation, brain water content was reduced from (84.48±1.802){\%} to (75.58±1.559){\%} (P=0.004), the expression of IL-1β and TNF-α near injury area also decreased(P=0.000 6 and 0.000 3), as well as the expression of ROS(P=0.020). The fluorescence intensity of activated astrocytes decreased from (2 906±431.591)to (165 8±312.912) (P=0.041), and the number of microglias and activated microglias were both reduced(P=0.049 and P<0.01) after TBI. Conclusions Wharton's jelly alleviated the inflammation and depression in traumatic brain injured mice.",
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author = "Tian Cheng and Wenwen Liu and Shanshan Ma and Tongwen Sun and Fangxia Guan",
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T1 - Transplantation of Wharton's jelly alleviates inflammatory response and depression after traumatic brain injury in mice

AU - Cheng, Tian

AU - Liu, Wenwen

AU - Ma, Shanshan

AU - Sun, Tongwen

AU - Guan, Fangxia

PY - 2018/7/1

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N2 - Objective To investigate the influence of Wharton's jelly (WJ) transplant on brain inflammation and mood status in traumatic brain injury (TBI) mouse model. Methods The WJ was isolated from human umbilical cord and cultured, and the cell phenotype of P3 human umbilical cord mesenchymal stem cells was identified by flow cytometry.The animal model was established by modified weight drop method. Experimental mice were randomly(random number) divided into Veh(normal saline) and WJ transplantation groups. After 3 days, water content of damaged brain was detected. Elisa kit was used to detect the expression of IL-1 p and TNF-a. The expression of GFAP, Ibal and CD68 were detected by immunofluorescence. Het(hydroethidine) staining was used to detect reactive oxygen species (ROS) production. Neurologic deficit score was used to evaluate the motor function, sucrose preference test, tail suspension test and forced swim test were used to detect the depression of mice.The data were expressed in (x±s) and analysēd by SPSS 21.0 software. Two-way ANOVA with repeated measures design was used to compare difference bewteen two groups at multiple interval. Results Compared with Veh group, the mice in the WJ group had a better performance in NDS scored test(dl: 14.8±1.169 vs. 15.2±1.472); 3d: (U.0±1.414 vs. 13.5±1.225); 7d: (9.5±1.517 vs. 12.0±1.549); 14d: (7.7±0.816 vs. 10.5±1.643); 21d: (6.5±0.547 vs. 9.0±1.265); 28d: (5.3±0.816 vs. 7.8±1.169), P<0.05]. After TBI, WJ tissue transplantation increased sucrose preference index from (54.49± 1.505)% to (64.56±2.279)% (P=0.004), decreased immobility time using tail suspension test from (144.7±5.493)s to (115.7±4.660)s (P<0.01), and decreased immobility time using forced swim test from (260.3±4.558)s to (215.8±5.003)s (P=0.002). After WJ transplantation, brain water content was reduced from (84.48±1.802)% to (75.58±1.559)% (P=0.004), the expression of IL-1β and TNF-α near injury area also decreased(P=0.000 6 and 0.000 3), as well as the expression of ROS(P=0.020). The fluorescence intensity of activated astrocytes decreased from (2 906±431.591)to (165 8±312.912) (P=0.041), and the number of microglias and activated microglias were both reduced(P=0.049 and P<0.01) after TBI. Conclusions Wharton's jelly alleviated the inflammation and depression in traumatic brain injured mice.

AB - Objective To investigate the influence of Wharton's jelly (WJ) transplant on brain inflammation and mood status in traumatic brain injury (TBI) mouse model. Methods The WJ was isolated from human umbilical cord and cultured, and the cell phenotype of P3 human umbilical cord mesenchymal stem cells was identified by flow cytometry.The animal model was established by modified weight drop method. Experimental mice were randomly(random number) divided into Veh(normal saline) and WJ transplantation groups. After 3 days, water content of damaged brain was detected. Elisa kit was used to detect the expression of IL-1 p and TNF-a. The expression of GFAP, Ibal and CD68 were detected by immunofluorescence. Het(hydroethidine) staining was used to detect reactive oxygen species (ROS) production. Neurologic deficit score was used to evaluate the motor function, sucrose preference test, tail suspension test and forced swim test were used to detect the depression of mice.The data were expressed in (x±s) and analysēd by SPSS 21.0 software. Two-way ANOVA with repeated measures design was used to compare difference bewteen two groups at multiple interval. Results Compared with Veh group, the mice in the WJ group had a better performance in NDS scored test(dl: 14.8±1.169 vs. 15.2±1.472); 3d: (U.0±1.414 vs. 13.5±1.225); 7d: (9.5±1.517 vs. 12.0±1.549); 14d: (7.7±0.816 vs. 10.5±1.643); 21d: (6.5±0.547 vs. 9.0±1.265); 28d: (5.3±0.816 vs. 7.8±1.169), P<0.05]. After TBI, WJ tissue transplantation increased sucrose preference index from (54.49± 1.505)% to (64.56±2.279)% (P=0.004), decreased immobility time using tail suspension test from (144.7±5.493)s to (115.7±4.660)s (P<0.01), and decreased immobility time using forced swim test from (260.3±4.558)s to (215.8±5.003)s (P=0.002). After WJ transplantation, brain water content was reduced from (84.48±1.802)% to (75.58±1.559)% (P=0.004), the expression of IL-1β and TNF-α near injury area also decreased(P=0.000 6 and 0.000 3), as well as the expression of ROS(P=0.020). The fluorescence intensity of activated astrocytes decreased from (2 906±431.591)to (165 8±312.912) (P=0.041), and the number of microglias and activated microglias were both reduced(P=0.049 and P<0.01) after TBI. Conclusions Wharton's jelly alleviated the inflammation and depression in traumatic brain injured mice.

KW - Depression

KW - Inflammation

KW - Traumatic brain injury

KW - Wharton's jelly

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