Transplantability of a circadian clock to a noncircadian organism

Anna H. Chen, David Lubkowicz, Vivian Yeong, Roger L. Chang, Pamela A. Silver

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Circadian oscillators are posttranslationally regulated and affect gene expression in autotrophic cyanobacteria. Oscillations are controlled by phosphorylation of the KaiC protein, which is modulated by the KaiA and KaiB proteins. However, it remains unclear how time information is transmitted to transcriptional output. We show reconstruction of the KaiABC oscillator in the noncircadian bacterium Escherichia coli. This orthogonal system shows circadian oscillations in KaiC phosphorylation and in a synthetic transcriptional reporter. Coexpression of KaiABC with additional native cyanobacterial components demonstrates a minimally sufficient set of proteins for transcriptional output from a native cyanobacterial promoter in E. coli. Together, these results demonstrate that a circadian oscillator is transplantable to a heterologous organism for reductive study as well as wide-ranging applications.

Original languageEnglish (US)
Article numbere1500358
JournalScience Advances
Volume1
Issue number5
DOIs
StatePublished - Jun 2015
Externally publishedYes

ASJC Scopus subject areas

  • General

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