Transmembrane movement of cholesterol in small unilamellar vesicles detected by cholesterol oxidase.

J. M. Backer, E. A. Dawidowicz

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Greater than 90% of the cholesterol in small unilamellar vesicles composed of egg lecithin and cholesterol (molar ratio 1:0.7) was oxidized by a cholesterol oxidase from Brevibacterium sp., with a single time constant and a half-time of 1 min at 37 degrees C. The enzyme preparation used was at least 95% pure and possessed no detectable phospholipase C activity. Since cholesterol is present in both halves of the bilayer, it was concluded that transmembrane movement of cholesterol in these vesicles occurs with a half-time of 1 min or less at 37 degrees C.

Original languageEnglish (US)
Pages (from-to)586-588
Number of pages3
JournalJournal of Biological Chemistry
Volume256
Issue number2
StatePublished - Jan 25 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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