Greater than 90% of the cholesterol in small unilamellar vesicles composed of egg lecithin and cholesterol (molar ratio 1:0.7) was oxidized by a cholesterol oxidase from Brevibacterium sp., with a single time constant and a half-time of 1 min at 37 degrees C. The enzyme preparation used was at least 95% pure and possessed no detectable phospholipase C activity. Since cholesterol is present in both halves of the bilayer, it was concluded that transmembrane movement of cholesterol in these vesicles occurs with a half-time of 1 min or less at 37 degrees C.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of Biological Chemistry|
|State||Published - Jan 25 1981|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology