Translocation of the catalytic domain of diphtheria toxin across planar phospholipid bilayers by its own T domain

Kyoung Joon Oh, Lisa Senzel, R. John Collier, Alan Finkelstein

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

The T domain of diphtheria toxin is known to participate in the pH- dependent translocation of the catalytic C domain of the toxin across the endosomal membrane, but how it does so, and whether cellular proteins are also required for this process, remain unknown. Here, we report results showing that the T domain alone is capable of translocating the entire C domain across model, planar phospholipid bilayers in the absence of other proteins. The T domain therefore contains the entire molecular machinery for mediating transfer of the catalytic domain of diphtheria toxin across membranes.

Original languageEnglish (US)
Pages (from-to)8467-8470
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number15
DOIs
StatePublished - Jul 20 1999

ASJC Scopus subject areas

  • General

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