Translocation of inserted foreign epitopes by a channel-forming protein

Karen S. Jakes, Paul K. Kienker, Stephen L. Slatin, Alan Finkelstein

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Certain bacterial protein toxins are able to insert themselves into, and at least partially across, lipid bilayer membranes in the absence of any auxiliary proteins, by using unknown mechanisms to overcome the high energy harrier presented by the hydrophobic bilayer core. We have previously shown that one such toxin, colicin Ia, translocates a large, hydrophilic part of itself completely across a lipid bilayer in conjunction with the formation of an ion-conducting channel. To address the question of whether the colicin can translocate any arbitrary amino acid sequence, we have altered the translocated segment by inserting, singly, two different foreign epitopes. Colicins containing either epitope retain significant bactericidal activity and form channels of normal conductance in planar bilayers. Furthermore, antibodies added on the side of the bilayer opposite that to which the colicin was added interact specifically with the corresponding epitopes, producing an inhibition of channel closing. Thus, the inserted epitopes are translocated along with the rest of the segment, suggesting that a surprisingly small part of colicin Ia, located elsewhere in the molecule, acts as a nonspecific protein translocator.

Original languageEnglish (US)
Pages (from-to)4321-4326
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number8
DOIs
StatePublished - Apr 14 1998

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Colicins
Epitopes
Lipid Bilayers
Proteins
Bacterial Toxins
Bacterial Proteins
Ion Channels
Amino Acid Sequence
Membranes
Antibodies

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Translocation of inserted foreign epitopes by a channel-forming protein. / Jakes, Karen S.; Kienker, Paul K.; Slatin, Stephen L.; Finkelstein, Alan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 8, 14.04.1998, p. 4321-4326.

Research output: Contribution to journalArticle

Jakes, Karen S. ; Kienker, Paul K. ; Slatin, Stephen L. ; Finkelstein, Alan. / Translocation of inserted foreign epitopes by a channel-forming protein. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 8. pp. 4321-4326.
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