Transition state variation in enzymatic reactions

Vern L. Schramm

doi:10.1016/S1367-5931(00)00246-5

Transition state variation in enzymatic reactions

Vern L. Schramm

Biochemistry

Research output: Contribution to journal › Review article › peer-review

25 Scopus citations

Abstract

Experimental analysis of enzymatic transition states by kinetic isotope effect methods has established geometric variation in related transition state structures. Differences are apparent in development of the reaction coordinate, in solvolytic transition states relative to those in enzymatic catalytic sites, in the stereochemistry of related substrates at the transition state, and in reactions catalyzed by related enzymes.

Original language	English (US)
Pages (from-to)	556-564
Number of pages	9
Journal	Current Opinion in Chemical Biology
Volume	5
Issue number	5
DOIs	https://doi.org/10.1016/S1367-5931(00)00246-5
State	Published - Oct 1 2001

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1016/S1367-5931(00)00246-5

Cite this

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title = "Transition state variation in enzymatic reactions",

abstract = "Experimental analysis of enzymatic transition states by kinetic isotope effect methods has established geometric variation in related transition state structures. Differences are apparent in development of the reaction coordinate, in solvolytic transition states relative to those in enzymatic catalytic sites, in the stereochemistry of related substrates at the transition state, and in reactions catalyzed by related enzymes.",

author = "Schramm, {Vern L.}",

note = "Funding Information: This work was supported by research grants from the National Institutes of Health. The author thanks Mr Brett Lewis and Dr Setu Roday for their comments; and the many associates listed in the references who have contributed to our knowledge of enzymatic transition states. ",

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N1 - Funding Information: This work was supported by research grants from the National Institutes of Health. The author thanks Mr Brett Lewis and Dr Setu Roday for their comments; and the many associates listed in the references who have contributed to our knowledge of enzymatic transition states.

PY - 2001/10/1

Y1 - 2001/10/1

N2 - Experimental analysis of enzymatic transition states by kinetic isotope effect methods has established geometric variation in related transition state structures. Differences are apparent in development of the reaction coordinate, in solvolytic transition states relative to those in enzymatic catalytic sites, in the stereochemistry of related substrates at the transition state, and in reactions catalyzed by related enzymes.

AB - Experimental analysis of enzymatic transition states by kinetic isotope effect methods has established geometric variation in related transition state structures. Differences are apparent in development of the reaction coordinate, in solvolytic transition states relative to those in enzymatic catalytic sites, in the stereochemistry of related substrates at the transition state, and in reactions catalyzed by related enzymes.

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DO - 10.1016/S1367-5931(00)00246-5

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Transition state variation in enzymatic reactions

Abstract

ASJC Scopus subject areas

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