Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins

Meng Chiao Ho, Matthew B. Sturm, Steven C. Almo, Vern L. Schramm

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Ricin A-chain (RTA) and saporin-L1 (SAP) catalyze adenosine depurination of 28S rRNA to inhibit protein synthesis and cause cell death. We present the crystal structures of RTA and SAP in complex with transition state analogue inhibitors. These tight-binding inhibitors mimic the sarcin-ricin recognition loop of 28S rRNA and the dissociative ribocation transition state established for RTA catalysis. RTA and SAP share unique purine-binding geometry with quadruple π-stacking interactions between adjacent adenine and guanine bases and 2 conserved tyrosines. An arginine at one end of the π-stack provides cationic polarization and enhanced leaving group ability to the susceptible adenine. Common features of these ribosome-inactivating proteins include adenine leaving group activation, a remarkable lack of ribocation stabilization, and conserved glutamates as general bases for activation of the H2O nucleophile. Catalytic forces originate primarily from leaving group activation evident in both RTA and SAP in complex with transition state analogues.

Original languageEnglish (US)
Pages (from-to)20276-20281
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number48
DOIs
Publication statusPublished - Dec 1 2009

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Keywords

  • Depurination
  • Ricin toxin A-chain
  • Saporin-L1
  • Sarcin-ricin loop

ASJC Scopus subject areas

  • General

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