TY - JOUR
T1 - Transgenic mouse models for studying mutations in vivo
T2 - Applications in aging research
AU - Vijg, Jan
AU - Dollé, Martijn E.T.
AU - Martus, Hans Jörg
AU - Boerrigter, Michael E.T.I.
N1 - Funding Information:
This work was supported by NIH grants P01 1801 AG10829-01, 1 P30 AG13314-01 and 1 RO1 ES/CA 08797-01, Dr J. Vijg is an awardee of the Naito Foundation.
PY - 1997/12
Y1 - 1997/12
N2 - To study mutation accumulation in the DNA of somatic cells and tissues during aging in vivo, a transgenic mouse model has been constructed. The model harbors plasmid vectors, containing the lacZ reporter gene, integrated head to tail at various chromosomal locations. Procedures have been worked out to efficiently recover the plasmids into E. coli host cells. A positive selection system, permitting only E. coli cells with a lacZ mutated plasmid to grow, allows for the accurate determination of mutation frequencies as the ratio of mutant colonies versus the total number of transformants, i.e., the total number of plasmid copies recovered. Results obtained from a life span study of plasmid mice with vector clusters on chromosome 3 and 4 indicated age-related mutation accumulation in the liver, but not in the brain. Comparison of the mutational spectra revealed a significantly larger proportion of large size-change mutations in liver than in brain.
AB - To study mutation accumulation in the DNA of somatic cells and tissues during aging in vivo, a transgenic mouse model has been constructed. The model harbors plasmid vectors, containing the lacZ reporter gene, integrated head to tail at various chromosomal locations. Procedures have been worked out to efficiently recover the plasmids into E. coli host cells. A positive selection system, permitting only E. coli cells with a lacZ mutated plasmid to grow, allows for the accurate determination of mutation frequencies as the ratio of mutant colonies versus the total number of transformants, i.e., the total number of plasmid copies recovered. Results obtained from a life span study of plasmid mice with vector clusters on chromosome 3 and 4 indicated age-related mutation accumulation in the liver, but not in the brain. Comparison of the mutational spectra revealed a significantly larger proportion of large size-change mutations in liver than in brain.
KW - Aging
KW - Mutation
KW - Transgenic mice
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U2 - 10.1016/S0047-6374(97)00107-3
DO - 10.1016/S0047-6374(97)00107-3
M3 - Article
C2 - 9352489
AN - SCOPUS:0030692613
SN - 0047-6374
VL - 98
SP - 189
EP - 202
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 3
ER -