Transgenic mice encoding modern imaging probes: Properties and applications

Ludmila A. Kasatkina, Vladislav V. Verkhusha

Research output: Contribution to journalReview articlepeer-review

Abstract

Modern biology is increasingly reliant on optical technologies, including visualization and longitudinal monitoring of cellular processes. The major limitation here is the availability of animal models to track the molecules and cells in their natural environment in vivo. Owing to the integrity of the studied tissue and the high stability of transgene expression throughout life, transgenic mice encoding fluorescent proteins and biosensors represent unique tools for in vivo studies in norm and pathology. We review the strategies for targeting probe expression in specific tissues, cell subtypes, or cellular compartments. We describe the application of transgenic mice expressing fluorescent proteins for tracking protein expression patterns, apoptotic events, tissue differentiation and regeneration, neurogenesis, tumorigenesis, and cell fate mapping. We overview the possibilities of functional imaging of secondary messengers, neurotransmitters, and ion fluxes. Finally, we provide the rationale and perspectives for the use of transgenic imaging probes in translational research and drug discovery.

Original languageEnglish (US)
Article number110845
JournalCell Reports
Volume39
Issue number8
DOIs
StatePublished - May 24 2022

Keywords

  • activity-dependent promoter
  • biosensor
  • cell-specific promoter
  • conditional expression
  • CP: Cell biology
  • CP: Neuroscience
  • Cre/loxP recombination
  • fluorescent protein
  • in vivo imaging

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'Transgenic mice encoding modern imaging probes: Properties and applications'. Together they form a unique fingerprint.

Cite this