TY - JOUR
T1 - Transgenic mice encoding modern imaging probes
T2 - Properties and applications
AU - Kasatkina, Ludmila A.
AU - Verkhusha, Vladislav V.
N1 - Funding Information:
Supported by grants GM122567 and NS115581 from the National Institutes of Health and 226178 from the Chan Zuckerberg Initiative .
Publisher Copyright:
© 2022 The Author(s)
PY - 2022/5/24
Y1 - 2022/5/24
N2 - Modern biology is increasingly reliant on optical technologies, including visualization and longitudinal monitoring of cellular processes. The major limitation here is the availability of animal models to track the molecules and cells in their natural environment in vivo. Owing to the integrity of the studied tissue and the high stability of transgene expression throughout life, transgenic mice encoding fluorescent proteins and biosensors represent unique tools for in vivo studies in norm and pathology. We review the strategies for targeting probe expression in specific tissues, cell subtypes, or cellular compartments. We describe the application of transgenic mice expressing fluorescent proteins for tracking protein expression patterns, apoptotic events, tissue differentiation and regeneration, neurogenesis, tumorigenesis, and cell fate mapping. We overview the possibilities of functional imaging of secondary messengers, neurotransmitters, and ion fluxes. Finally, we provide the rationale and perspectives for the use of transgenic imaging probes in translational research and drug discovery.
AB - Modern biology is increasingly reliant on optical technologies, including visualization and longitudinal monitoring of cellular processes. The major limitation here is the availability of animal models to track the molecules and cells in their natural environment in vivo. Owing to the integrity of the studied tissue and the high stability of transgene expression throughout life, transgenic mice encoding fluorescent proteins and biosensors represent unique tools for in vivo studies in norm and pathology. We review the strategies for targeting probe expression in specific tissues, cell subtypes, or cellular compartments. We describe the application of transgenic mice expressing fluorescent proteins for tracking protein expression patterns, apoptotic events, tissue differentiation and regeneration, neurogenesis, tumorigenesis, and cell fate mapping. We overview the possibilities of functional imaging of secondary messengers, neurotransmitters, and ion fluxes. Finally, we provide the rationale and perspectives for the use of transgenic imaging probes in translational research and drug discovery.
KW - CP: Cell biology
KW - CP: Neuroscience
KW - Cre/loxP recombination
KW - activity-dependent promoter
KW - biosensor
KW - cell-specific promoter
KW - conditional expression
KW - fluorescent protein
KW - in vivo imaging
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U2 - 10.1016/j.celrep.2022.110845
DO - 10.1016/j.celrep.2022.110845
M3 - Review article
C2 - 35613592
AN - SCOPUS:85130555907
VL - 39
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 8
M1 - 110845
ER -