Transforming growth factor a1 and extracellular matrix protease expression in the uterosacral ligaments of patients with and without pelvic organ prolapse

Ava Leegant, Lisa C. Zuckerwise, Keith Downing, Jurriaan Brouwer-Visser, Changcheng Zhu, Maria Jose Cossio, Felix Strube, Xianhong Xie, Erika Banks, Gloria S. Huang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Objectives: This study was undertaken to evaluate the expression of transforming growth factor β1 (TGF-β1) and matrix metalloproteinase 9 (MMP-9), key regulators of the extracellular matrix composition, in the uterosacral ligaments (USLs) of women with pelvic organ prolapse (POP) compared with controls. Methods: Under an institutional review board approval, USL samples were obtained from women undergoing vaginal hysterectomy for stage 2 or greater POP (cases, n = 21) and from women without POP undergoing vaginal hysterectomy for benign indications (controls, n = 19). Hematoxylin and eosin and trichrome staining were performed on the USL sections, and the distribution of smooth muscle and fibrous tissue were quantified. Immunohistochemical staining was performed using anti-TGF-β1 and anti-MMP-9 antibodies. The expressions of TGF-β1 and MMP-9 were evaluated by the pathologist, who was blinded to all clinical data. Results: Transforming growth factor β1 expression positively correlated with MMP-9 expression (R = 0.4, P = 0.01). The expressions of TGF-β1 and MMP-9 were similar in subjects with POP versus controls. There was a significant increase in fibrous tissue (P = 0.008) and a corresponding decrease in smooth muscle (P = 0.03), associated with increasing age. The TGF-β1 expression, but not MMP-9 expression, also significantly increased with age (P = 0.02). Discussion: Although our study uncovered age-related alterations in USL composition and TGF-β1 expression, there was no difference in the expression of TGF-β1 or MMP-9 in the subjects with POP versus controls.

Original languageEnglish (US)
Pages (from-to)53-58
Number of pages6
JournalFemale Pelvic Medicine and Reconstructive Surgery
Volume21
Issue number1
DOIs
StatePublished - Jan 1 2015

Keywords

  • MMP-9
  • Pelvic organ prolapse
  • TGF-β1
  • Uterosacral ligament

ASJC Scopus subject areas

  • Surgery
  • Obstetrics and Gynecology
  • Urology

Fingerprint Dive into the research topics of 'Transforming growth factor a1 and extracellular matrix protease expression in the uterosacral ligaments of patients with and without pelvic organ prolapse'. Together they form a unique fingerprint.

  • Cite this