Transforming growth factor a1 and extracellular matrix protease expression in the uterosacral ligaments of patients with and without pelvic organ prolapse

Ava R. Leegant, Lisa C. Zuckerwise, Keith Downing, Jurriaan Brouwer-Visser, Changcheng Zhu, Maria Jose Cossio, Felix Strube, Xianhong Xie, Erika Banks, Gloria S. Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: This study was undertaken to evaluate the expression of transforming growth factor β1 (TGF-β1) and matrix metalloproteinase 9 (MMP-9), key regulators of the extracellular matrix composition, in the uterosacral ligaments (USLs) of women with pelvic organ prolapse (POP) compared with controls. Methods: Under an institutional review board approval, USL samples were obtained from women undergoing vaginal hysterectomy for stage 2 or greater POP (cases, n = 21) and from women without POP undergoing vaginal hysterectomy for benign indications (controls, n = 19). Hematoxylin and eosin and trichrome staining were performed on the USL sections, and the distribution of smooth muscle and fibrous tissue were quantified. Immunohistochemical staining was performed using anti-TGF-β1 and anti-MMP-9 antibodies. The expressions of TGF-β1 and MMP-9 were evaluated by the pathologist, who was blinded to all clinical data. Results: Transforming growth factor β1 expression positively correlated with MMP-9 expression (R = 0.4, P = 0.01). The expressions of TGF-β1 and MMP-9 were similar in subjects with POP versus controls. There was a significant increase in fibrous tissue (P = 0.008) and a corresponding decrease in smooth muscle (P = 0.03), associated with increasing age. The TGF-β1 expression, but not MMP-9 expression, also significantly increased with age (P = 0.02). Discussion: Although our study uncovered age-related alterations in USL composition and TGF-β1 expression, there was no difference in the expression of TGF-β1 or MMP-9 in the subjects with POP versus controls.

Original languageEnglish (US)
Pages (from-to)53-58
Number of pages6
JournalFemale Pelvic Medicine and Reconstructive Surgery
Volume21
Issue number1
DOIs
StatePublished - 2015

Fingerprint

Pelvic Organ Prolapse
Transforming Growth Factors
Ligaments
Matrix Metalloproteinase 9
Extracellular Matrix
Peptide Hydrolases
Vaginal Hysterectomy
Smooth Muscle
Staining and Labeling
Research Ethics Committees
Hematoxylin
Eosine Yellowish-(YS)
Muscles
Antibodies

Keywords

  • MMP-9
  • Pelvic organ prolapse
  • TGF-β1
  • Uterosacral ligament

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Surgery
  • Urology

Cite this

Transforming growth factor a1 and extracellular matrix protease expression in the uterosacral ligaments of patients with and without pelvic organ prolapse. / Leegant, Ava R.; Zuckerwise, Lisa C.; Downing, Keith; Brouwer-Visser, Jurriaan; Zhu, Changcheng; Cossio, Maria Jose; Strube, Felix; Xie, Xianhong; Banks, Erika; Huang, Gloria S.

In: Female Pelvic Medicine and Reconstructive Surgery, Vol. 21, No. 1, 2015, p. 53-58.

Research output: Contribution to journalArticle

@article{a72c5b5a8a3548889ffea04e9c34f7b2,
title = "Transforming growth factor a1 and extracellular matrix protease expression in the uterosacral ligaments of patients with and without pelvic organ prolapse",
abstract = "Objectives: This study was undertaken to evaluate the expression of transforming growth factor β1 (TGF-β1) and matrix metalloproteinase 9 (MMP-9), key regulators of the extracellular matrix composition, in the uterosacral ligaments (USLs) of women with pelvic organ prolapse (POP) compared with controls. Methods: Under an institutional review board approval, USL samples were obtained from women undergoing vaginal hysterectomy for stage 2 or greater POP (cases, n = 21) and from women without POP undergoing vaginal hysterectomy for benign indications (controls, n = 19). Hematoxylin and eosin and trichrome staining were performed on the USL sections, and the distribution of smooth muscle and fibrous tissue were quantified. Immunohistochemical staining was performed using anti-TGF-β1 and anti-MMP-9 antibodies. The expressions of TGF-β1 and MMP-9 were evaluated by the pathologist, who was blinded to all clinical data. Results: Transforming growth factor β1 expression positively correlated with MMP-9 expression (R = 0.4, P = 0.01). The expressions of TGF-β1 and MMP-9 were similar in subjects with POP versus controls. There was a significant increase in fibrous tissue (P = 0.008) and a corresponding decrease in smooth muscle (P = 0.03), associated with increasing age. The TGF-β1 expression, but not MMP-9 expression, also significantly increased with age (P = 0.02). Discussion: Although our study uncovered age-related alterations in USL composition and TGF-β1 expression, there was no difference in the expression of TGF-β1 or MMP-9 in the subjects with POP versus controls.",
keywords = "MMP-9, Pelvic organ prolapse, TGF-β1, Uterosacral ligament",
author = "Leegant, {Ava R.} and Zuckerwise, {Lisa C.} and Keith Downing and Jurriaan Brouwer-Visser and Changcheng Zhu and Cossio, {Maria Jose} and Felix Strube and Xianhong Xie and Erika Banks and Huang, {Gloria S.}",
year = "2015",
doi = "10.1097/SPV.0000000000000130",
language = "English (US)",
volume = "21",
pages = "53--58",
journal = "Female Pelvic Medicine and Reconstructive Surgery",
issn = "2151-8378",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Transforming growth factor a1 and extracellular matrix protease expression in the uterosacral ligaments of patients with and without pelvic organ prolapse

AU - Leegant, Ava R.

AU - Zuckerwise, Lisa C.

AU - Downing, Keith

AU - Brouwer-Visser, Jurriaan

AU - Zhu, Changcheng

AU - Cossio, Maria Jose

AU - Strube, Felix

AU - Xie, Xianhong

AU - Banks, Erika

AU - Huang, Gloria S.

PY - 2015

Y1 - 2015

N2 - Objectives: This study was undertaken to evaluate the expression of transforming growth factor β1 (TGF-β1) and matrix metalloproteinase 9 (MMP-9), key regulators of the extracellular matrix composition, in the uterosacral ligaments (USLs) of women with pelvic organ prolapse (POP) compared with controls. Methods: Under an institutional review board approval, USL samples were obtained from women undergoing vaginal hysterectomy for stage 2 or greater POP (cases, n = 21) and from women without POP undergoing vaginal hysterectomy for benign indications (controls, n = 19). Hematoxylin and eosin and trichrome staining were performed on the USL sections, and the distribution of smooth muscle and fibrous tissue were quantified. Immunohistochemical staining was performed using anti-TGF-β1 and anti-MMP-9 antibodies. The expressions of TGF-β1 and MMP-9 were evaluated by the pathologist, who was blinded to all clinical data. Results: Transforming growth factor β1 expression positively correlated with MMP-9 expression (R = 0.4, P = 0.01). The expressions of TGF-β1 and MMP-9 were similar in subjects with POP versus controls. There was a significant increase in fibrous tissue (P = 0.008) and a corresponding decrease in smooth muscle (P = 0.03), associated with increasing age. The TGF-β1 expression, but not MMP-9 expression, also significantly increased with age (P = 0.02). Discussion: Although our study uncovered age-related alterations in USL composition and TGF-β1 expression, there was no difference in the expression of TGF-β1 or MMP-9 in the subjects with POP versus controls.

AB - Objectives: This study was undertaken to evaluate the expression of transforming growth factor β1 (TGF-β1) and matrix metalloproteinase 9 (MMP-9), key regulators of the extracellular matrix composition, in the uterosacral ligaments (USLs) of women with pelvic organ prolapse (POP) compared with controls. Methods: Under an institutional review board approval, USL samples were obtained from women undergoing vaginal hysterectomy for stage 2 or greater POP (cases, n = 21) and from women without POP undergoing vaginal hysterectomy for benign indications (controls, n = 19). Hematoxylin and eosin and trichrome staining were performed on the USL sections, and the distribution of smooth muscle and fibrous tissue were quantified. Immunohistochemical staining was performed using anti-TGF-β1 and anti-MMP-9 antibodies. The expressions of TGF-β1 and MMP-9 were evaluated by the pathologist, who was blinded to all clinical data. Results: Transforming growth factor β1 expression positively correlated with MMP-9 expression (R = 0.4, P = 0.01). The expressions of TGF-β1 and MMP-9 were similar in subjects with POP versus controls. There was a significant increase in fibrous tissue (P = 0.008) and a corresponding decrease in smooth muscle (P = 0.03), associated with increasing age. The TGF-β1 expression, but not MMP-9 expression, also significantly increased with age (P = 0.02). Discussion: Although our study uncovered age-related alterations in USL composition and TGF-β1 expression, there was no difference in the expression of TGF-β1 or MMP-9 in the subjects with POP versus controls.

KW - MMP-9

KW - Pelvic organ prolapse

KW - TGF-β1

KW - Uterosacral ligament

UR - http://www.scopus.com/inward/record.url?scp=84927613443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927613443&partnerID=8YFLogxK

U2 - 10.1097/SPV.0000000000000130

DO - 10.1097/SPV.0000000000000130

M3 - Article

C2 - 25185601

AN - SCOPUS:84927613443

VL - 21

SP - 53

EP - 58

JO - Female Pelvic Medicine and Reconstructive Surgery

JF - Female Pelvic Medicine and Reconstructive Surgery

SN - 2151-8378

IS - 1

ER -