Transformation of cells of astrocyte lineage into macrophage-like cells in organotypic cultures of mouse spinal cord tissue

H. Kusaka, A. Hirano, M. B. Bornstein, G. R W Moore, C. S. Raine

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Phagocytic cells on the surface of the explants and their relationships to the surface were examined morphologically and immunocytochemically in organotypic cultures of mouse spinal cord tissue. Phagocytic cells were rounded, had smooth cytoplasmic surfaces and were occasionally closely apposed to underlying cells by junctional complexes. These cells contained dense bodies, vacuoles, smooth and coated vesicles, a few microtubules and bundles of intermediate filaments similar to astroglial filaments. The superficial layer of the explant which usually consisted of astroglial cell bodies and their processes, sometimes contained immature neuroepithelial cells with numerous free ribosomes, centrioles, Golgi apparatus, microtubules and infrequently, intermediate filaments. Overall, the cells resembled poorly differentiated astrocytes. Numerous dense bodies and coated vesicles were observed in some of these immature cells as well as in astrocytes in the surface layer of the explant. Cytoplasmic bridges between immature cells within the explant and phagocytic cells on the surface were observed. Immunocytochemistry revealed the presence of glial fibrillary acidic protein within these surface phagocytic cells. It thus appears that immature neuroepithelial cells of astrocytic lineage are capable of transforming into macrophage-like cells in organotypic culture.

Original languageEnglish (US)
Pages (from-to)77-89
Number of pages13
JournalJournal of the Neurological Sciences
Volume72
Issue number1
DOIs
StatePublished - 1986

Fingerprint

Cell Lineage
Astrocytes
Spinal Cord
Macrophages
Phagocytes
Neuroepithelial Cells
Coated Vesicles
Intermediate Filaments
Microtubules
Centrioles
Glial Fibrillary Acidic Protein
Golgi Apparatus
Vacuoles
Ribosomes
Immunohistochemistry

Keywords

  • Astrocyte
  • Epithelial cell
  • Macrophage
  • Mouse organotypic culture
  • Phagocytosis
  • Spinal cord

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

Cite this

Transformation of cells of astrocyte lineage into macrophage-like cells in organotypic cultures of mouse spinal cord tissue. / Kusaka, H.; Hirano, A.; Bornstein, M. B.; Moore, G. R W; Raine, C. S.

In: Journal of the Neurological Sciences, Vol. 72, No. 1, 1986, p. 77-89.

Research output: Contribution to journalArticle

Kusaka, H. ; Hirano, A. ; Bornstein, M. B. ; Moore, G. R W ; Raine, C. S. / Transformation of cells of astrocyte lineage into macrophage-like cells in organotypic cultures of mouse spinal cord tissue. In: Journal of the Neurological Sciences. 1986 ; Vol. 72, No. 1. pp. 77-89.
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